Extraction, physicochemical properties, and antioxidant activity of natural melanin from Auricularia heimuer fermentation

Natural melanin from have numerous beneficial biological properties, which were used as a safe and healthy colorant in several industries. In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-sol...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in nutrition (Lausanne) Vol. 10; p. 1131542
Main Authors Ma, Yinpeng, Zhang, Piqi, Dai, Xiaodong, Yao, Xiuge, Zhou, Shuyang, Ma, Qingfang, Liu, Jianing, Tian, Shuang, Zhu, Jianan, Zhang, Jiechi, Kong, Xianghui, Bao, Yihong
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 17.02.2023
Subjects
antioxidant activities
Auricularia heimuer
HPLC
natural melanin
Nutrition
physicochemical properties
antioxidant activities
physicochemical properties
Auricularia heimuer
HPLC
natural melanin
Online AccessGet full text

Cover

More Information
Summary:Natural melanin from have numerous beneficial biological properties, which were used as a safe and healthy colorant in several industries. In this study, single-factor experiments, Box-Behnken design (BBD), and response surface methodology (RSM) were employed to investigate the effects of alkali-soluble pH, acid precipitation pH, and microwave time on the extraction yield of melanin (AHM) from fermentation. Ultraviolet-visible spectrum (UV-Vis), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), and high-performance liquid chromatography (HPLC) were used to analyze the extracted AHM. The solubility, stability, and antioxidant activities of AHM were also measured. The results showed that alkali-soluble pH, acid precipitation pH, and microwave time significantly affected the AHM yield, with the following optimized microwave-assisted extraction conditions: alkali-soluble pH of 12.3, acid precipitation pH of 3.1, and microwave time of 53 min, resulting in an AHM extraction yield of 0.4042%. AHM exhibited a strong absorption at 210 nm, similar to melanin from other sources. FT-IR spectroscopy also revealed that AHM exhibited the three characteristic absorption peaks of natural melanin. The HPLC chromatogram profile of AHM showed a single symmetrical elution peak with a 2.435 min retention time. AHM was highly soluble in alkali solution, insoluble in distilled water and organic solvents, and demonstrated strong DPPH, OH, and ABTS free radical scavenging activities. This study provides technical support to optimize AHM extraction for use in the medical and food industries.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
This article was submitted to Food Chemistry, a section of the journal Frontiers in Nutrition
Edited by: Guo-Qing Zhang, Beijing University of Agriculture, China
Reviewed by: Xueran Geng, Shanxi Agricultural University, China; Meng Li, Beijing Technology and Business University, China
ISSN:2296-861X
2296-861X
DOI:10.3389/fnut.2023.1131542