Cross-Clade Cytotoxic T Cell Response to Human Immunodeficiency Virus Type 1 Proteins among HLA Disparate North Americans and Thais

• Published in: The Journal of infectious diseases Vol. 178; no. 4; pp. 1040 - 1046
• Main Authors: Lynch, Julia A., deSouza, Mark, Robb, Merlin D., Markowitz, Lauri, Nitayaphan, Sorachai, Sapan, Christine V., Mann, Dean L., Birx, Deborah L., Cox, Josephine H.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.10.1998; University of Chicago Press
• Subjects: Alleles; Analysis of the immune response. Humoral and cellular immunity; Antigens; Asian People - genetics; Biological and medical sciences; Black or African American; Black People - genetics; Cross Reactions; Epitopes; Florida; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Frequency; HIV; HIV 1; HIV Antigens - genetics; HIV Antigens - immunology; HIV Infections - classification; HIV Infections - immunology; HIV-1 - classification; HIV-1 - immunology; HLA antigens; HLA Antigens - genetics; Human viral diseases; Humans; Immunobiology; Infectious diseases; Major Articles; Medical sciences; Organs and cells involved in the immune response; Racial Groups - genetics; Recombinant Proteins - immunology; Serotyping; T lymphocytes; T-Lymphocytes, Cytotoxic - immunology; Thailand; Vaccination; Vaccinia; Vaccinia virus - genetics; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viruses; White People - genetics; Thailand; Asia; North America; America; Florida; Human; Immunopathology; HLA-System; Immune response; Genetic variability; HIV-1 virus; Retroviridae; Cytotoxicity; AIDS; Cellular immunity; Vaccine; Immune deficiency; Lentivirus; Ethnic group; Protein; Infection; Virus; Phenotype; Viral disease; T-Lymphocyte; Cross reaction; Human immunodeficiency virus
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/515652

A globally effective vaccine will need to elicit cytotoxic T lymphocytes (CTL) capable of recognizing diverse human immunodeficiency virus type 1 (HIV-1) clades. Study of the cellular immune responses of HIV-1—infected persons may allow predictions to be made regarding useful vaccine antigen components. The frequency and magnitude of CTL responses to clade E and B Gag, Pol-RT, Env, and Nef proteins were compared in 12 HLA-characterized, clade E—infected Thais and in 10 clade B—infected North Americans using vaccinia recombinant constructs for protein expression. While responses were detected against all proteins, they were most frequent and cross-reactive to Gag in both groups. Pol-RT was recognized less frequently in Thais than North Americans. Cross-clade protein recognition was common but not uniformly present among these HLA-disparate individuals. Population-specific CTL data are needed to adequately prepare for vaccine trials outside of North America and Europe.