Podocyte Detachment in Type 2 Diabetic Nephropathy

Background: Glomerular podocyte number declines and urinary excretion of podocytes increases as kidney disease progresses in persons with type 2 diabetes mellitus (T2DM). Methods: Using high-power electron microscopy, we quantified podocyte detachment in T2DM. Results: We evaluated 106 glomeruli (ra...

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Published inAmerican journal of nephrology Vol. 33; no. Suppl 1; pp. 21 - 24
Main Authors Weil, E. Jennifer, Lemley, Kevin V., Yee, Berne, Lovato, Tracy, Richardson, Meghan, Myers, Bryan D., Nelson, Robert G.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland S. Karger AG 01.01.2011
Subjects
Adult
Arizona
Basement Membrane - pathology
Basement Membrane - ultrastructure
Cell Adhesion
Diabetes Mellitus, Type 2 - complications
Diabetes Mellitus, Type 2 - ethnology
Diabetic Nephropathies - ethnology
Diabetic Nephropathies - etiology
Diabetic Nephropathies - pathology
Female
Histological Techniques
Humans
Indians, North American
Male
Microscopy, Electron
Middle Aged
Original Report: Laboratory Investigation
Podocytes - pathology
Podocytes - ultrastructure
Arizona
Podocyte
Histology
Electron microscopy
Morphometry
Diabetic kidney disease
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Summary:Background: Glomerular podocyte number declines and urinary excretion of podocytes increases as kidney disease progresses in persons with type 2 diabetes mellitus (T2DM). Methods: Using high-power electron microscopy, we quantified podocyte detachment in T2DM. Results: We evaluated 106 glomeruli (range 1–6 per subject) from 40 Pima Indian subjects with T2DM enrolled in a clinical trial. On high-power electron micrographs, 35% of the subjects had no evidence of podocyte detachment. Among the remaining subjects, the median percentage of basement membrane with podocyte detachment was 0.62% (interquartile range = 0.32–1.52%). Conclusion: Podocyte detachment from the glomerular basement membrane has been described and measured in type 1 diabetes mellitus using a different method. We now document podocyte detachment microscopically and quantify it morphometrically in humans with T2DM. The findings offer quantitative histologic support to a potential mechanism for the functional impairment, and possibly the sclerosis of glomeruli, in diabetic glomerular injury.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISBN:9783805597678
3805597673
ISSN:0250-8095
1421-9670
1421-9670
DOI:10.1159/000327047