Immobilization of horseradish peroxidase on titanate nanowires for biosensing application
Horseradish peroxidase (HRP) was immobilized on titanate nanowires (TNW) to produce a peroxide biosensor. Nanowires were synthesized by a simple and cost-effective alkaline hydrothermal method, and characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectra,...
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Published in | Journal of applied electrochemistry Vol. 46; no. 1; pp. 17 - 25 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.01.2016
|
Subjects | |
Online Access | Get full text |
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Summary: | Horseradish peroxidase (HRP) was immobilized on titanate nanowires (TNW) to produce a peroxide biosensor. Nanowires were synthesized by a simple and cost-effective alkaline hydrothermal method, and characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectra, and N
2
physisorption. In this study, HRP was covalently immobilized on TNW using glutaraldehyde as crosslinking agent after surface treatment with (3-aminopropyl)trimethoxysilane. Electrochemical evaluation of the biosensor was performed by cyclic voltammetry, exhibiting a low reduction potential for H
2
O
2
, around −0.38 V versus Ag/AgCl at pH 7.0, and a low detection limit of 1.2 µmol L
−1
. TNW have demonstrated excellent performance as immobilizing support for the enzyme as the biosensor exhibited a storage stability up to 30 days, retaining 91 % of its activity. All together, these results show that the produced biosensor based on the HRP enzyme, and TNW is efficient for the determination of H
2
O
2
, showing a fast and sensitive response.
Graphical Abstract |
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ISSN: | 0021-891X 1572-8838 |
DOI: | 10.1007/s10800-015-0907-z |