Nano-liquid chromatography applied to enantiomers separation

• Published in: Journal of Chromatography A Vol. 1486; pp. 20 - 34
• Main Author: Fanali, Salvatore
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24.02.2017
• Subjects: agrochemicals; Amylose; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; antibiotics; Cellulose; Chiral; Chromatography, High Pressure Liquid; Chromatography, Liquid - methods; cost effectiveness; Cyclodextrins; Cyclodextrins - chemistry; Cyclodextrins - isolation & purification; Enantiomers; Glycopeptide antibiotics; glycopeptides; Glycopeptides - chemistry; Glycopeptides - isolation & purification; high performance liquid chromatography; medicine; Nano-LC; Nano-liquid chromatography; Nanotechnology - methods; pharmaceutical industry; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Selectors; Stereoisomerism; Teicoplanin; Vancomycin; Polysaccharides; Amylose; Chiral; Cellulose; Cyclodextrins; Nano-liquid chromatography; Vancomycin; Enantiomers; Nano-LC; Selectors; Teicoplanin; Glycopeptide antibiotics
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2016.10.028

•Features of nano-liquid chromatography are presented.•Principles of chiral separation are briefly discussed.•The main chiral selectors used in nano-LC are described utilizing monolithic and packed capillaries.•The most important applications of nano-LC to enantiomers separation are reported. This paper presents the state of the art concerning the separation of chiral compounds by means of nano-liquid chromatography (nano-LC). The enantiomers’ separation and determination are a subject of fundamental importance in various application fields such as pharmaceutical industry, biomedicine, food, agrochemical etc. Nano-LC is a miniaturized chromatographic technique offering some advantages over conventional ones such as low consumption of mobile phase, sample volume and amount of chiral stationary phase, reduced costs etc. This is reported in the first part of the paper illustrating the features of the nano-LC. In addition, chiral resolution methods are briefly illustrated. Some chiral selectors, used in high-performance liquid chromatography have also been applied in nano-LC including cyclodextrins, glycopeptide antibiotics, modified polysaccharides etc. This is discussed in the second part of the review. Finally some examples of the applications available in literature are reported.