Sequence and transcript analysis of the Nco2.5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids

• Published in: Molecular & general genetics Vol. 235; no. 2-3; p. 340
• Main Authors: Bonhomme, S. (Institut National de Recherches Agronomiques, Versailles (France). Lab. de Biologie Cellulaire), Budar, F, Lancelin, D, Small, I, Defrance, M.C, Pelletier, G
• Format: Journal Article
• Language: English
• Published: Germany 01.11.1992
• Subjects: Amino Acid Sequence; Base Sequence; BRASSICA; Brassica - genetics; Brassica - physiology; Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific; DNA, Mitochondrial - genetics; DNA, Mitochondrial - isolation & purification; Fertility - genetics; GENETICA MITOCHONDRIAL; GENETIQUE MITOCHONDRIALE; Genotype; HIBRIDOS; HYBRIDE; HYBRIDS; Mitochondrial DNA (mtDNA); MITOCHONDRIAL GENETICS; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Ogura; Open Reading Frames; orf138; orf158; Restriction Mapping; RNA - genetics; RNA - isolation & purification; RNA, Messenger - genetics; RNA, Transfer, Amino Acyl - genetics; RNA, Transfer, Met; SECUENCIA NUCLEICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; Transcription, Genetic
• ISSN: 0026-8925; 1432-1874
• DOI: 10.1007/BF00279379

Sequence analysis of the Ogura-specific mitochondrial DNA (mtDNA) fragment isolated previously from Brassica cybrids carrying Ogura cytoplasmic male sterility (cms) revealed a tRNA(fMet) sequence, a putative 138 amino acid open reading frame (orf138), and a 158 amino acid ORF (orf158) previously observed in mitochondrial genomes from several other plant species. Transcription mapping showed that both ORFs are present on a 1.4 kb cms-specific transcript. The orf158 sequence is also transcribed in fertile plants on a different mRNA, and thus is unlikely to be related to cms. On the other hand, fertile revertant plants lack transcripts of the orf138 sequence, whose possible role in the mechanism of Ogura cms is discussed.