State of art and best practices for fatty acid analysis in aquatic sciences

• Published in: ICES journal of marine science Vol. 77; no. 7-8; pp. 2375 - 2395
• Main Authors: Couturier, Lydie I E, Michel, Loïc N, Amaro, Teresa, Budge, Suzanne M, da Costa, Elisabete, De Troch, Marleen, Di Dato, Valeria, Fink, Patrick, Giraldo, Carolina, Le Grand, Fabienne, Loaiza, Iván, Mathieu-Resuge, Margaux, Nichols, Peter D, Parrish, Christopher C, Sardenne, Fany, Vagner, Marie, Pernet, Fabrice, Soudant, Philippe
• Format: Journal Article Web Resource
• Language: English
• Published: Oxford University Press 01.12.2020
• Subjects: Animal biology; aquatic nutrition; Aquatic sciences & oceanology; Biochemistry; Biochemistry, Molecular Biology; biomarkers; Environmental sciences & ecology; fatty acids; Life Sciences; marine and freshwater lipids; methods; recommended practices; Sciences aquatiques & océanologie; Sciences de l’environnement & écologie; Sciences du vivant; Zoologie; Zoology; fatty acids; recommended practices; aquatic nutrition; biomarkers; marine and freshwater lipids; methods; ACL
• ISSN: 1095-9289; 1054-3139; 1095-9289
• DOI: 10.1093/icesjms/fsaa121

Abstract Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.