Protection of BALB/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4

• Published in: American journal of veterinary research Vol. 57; no. 5; p. 677
• Main Authors: Winter, A.J. (Cornell University, Ithaca, NY.), Schurig, G.G, Boyle, S.M, Sriranganathan, N, Bevins, J.S, Enright, F.M, Elzer, P.H, Kopec, J.D
• Format: Journal Article
• Language: English
• Published: United States 01.05.1996
• Subjects: Alleles; Animals; Antibodies, Viral - analysis; Antibodies, Viral - immunology; Antigens, Viral - analysis; Antigens, Viral - immunology; Bison; Blotting, Western - veterinary; BRUCELLA; Brucella - genetics; Brucella - immunology; BRUCELLA MELITENSIS; Brucella melitensis - genetics; Brucella melitensis - immunology; Brucella Vaccine - immunology; BRUCELLOSE; BRUCELLOSIS; Brucellosis - immunology; Brucellosis - prevention & control; Brucellosis - veterinary; BRUCELOSIS; Cattle; CONTROL DE ENFERMEDADES; CONTROLE DE MALADIES; Deer; DISEASE CONTROL; DISEASE PREVENTION; EFFICACY; Female; INACTIVATED VACCINES; Listeria monocytogenes - immunology; LIVE VACCINES; MICE; Mice, Inbred BALB C - immunology; MUTANT; MUTANTES; MUTANTS; Mutation; RATON; Reindeer; Rodent Diseases - immunology; Rodent Diseases - prevention & control; SOURIS; Swine; VACCIN; VACCIN VIVANT; VACCINATION; VACCINES; Vaccines, Attenuated - pharmacology; VACUNA; VACUNA VIVA; VACUNACION
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.1996.57.05.677

To evaluate stable rough mutants derived from Brucella melitensis 16M and B suis 2579 (biovar 4) as vaccines against homologous and heterologous Brucella spp in the BALB/c mouse model. DESIGN, ANIMALS, AND PROCEDURE: Rough mutants VTRM1 and VTRS1 were obtained from B melitensis 16M and B suis 2579, respectively, by allelic exchange of rfbU gene encoding mannosyltransferase with a Tn5-disrupted rfbU gene. Mice were vaccinated with VTRM1 or VTRS1 and challenge exposed 8 weeks later. VTRM1 and VTRS1 replicated extensively in the spleen during the first 3 weeks of infection, then decreased rapidly. Antibodies specific for the O polysaccharide were not detected in sera of mice inoculated with either rough strain. Vaccination with VTRM1 or VTRS1 induced protection against virulent strains of B abortus (2308), B melitensis (16M), B suis biovar 1 (750), and B suis biovar 4 (2579). VTRM1 also protected against B ovis (PA) and against 4 field isolates of B abortus from bison or elk. VTRS1 conferred protection against 4 field isolates of B suis biovar 4 from reindeer. Vaccines prepared from live VTRM1 or VTRS1 provided significantly greater protection than that afforded by vaccines of killed cells in QS-21 adjuvant. Vaccination with VTRM1 containing VTRS1 gave minimal protection against the antigenically unrelated Listeria monocytogenes, thus demonstrating the immunologic specificity of protection against Brucella spp. Results encourage evaluation, in primary host species, of VTRM1 and VTRS1, along with RB51, as alternative vaccines to strain 19, Rev 1, or other smooth phase vaccines.