Genetic diversity analysis and transferability of cereal EST-SSR markers to orchardgrass ( Dactylis glomerata L.)

• Published in: Biochemical systematics and ecology Vol. 38; no. 4; pp. 740 - 749
• Main Authors: Xie, Wen-Gang, Zhang, Xin-Quan, Cai, Hong-Wei, Liu, Wei, Peng, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2010
• Subjects: AMOVA; Biochemistry; Dactylis glomerata; Dactylis glomerata L; Genetic diversity; SSR; Transferability; ANE, Europe; Genetic diversity; SSR; Transferability; Dactylis glomerata L; AMOVA
• ISSN: 0305-1978; 1873-2925
• DOI: 10.1016/j.bse.2010.06.009

The diversity and genetic relationships among 74 orchardgrass accessions were analyzed using cereal EST-SSRs and orchardgrass SSR markers in order to estimate genetic variability and compare the level of diversity. In total, 190 polymorphic bands were detected with an average of 6.3 alleles per SSR loci. The average polymorphic rate (P) for the species was 84.63%, suggesting a high degree of genetic diversity. The molecular variance analysis (AMOVA) showed that the proportion of variance explained by within- and among-geographical groups diversity was74.87% and 25.13%, respectively. The distinct geographical divergence of orchardgrass was revealed between Americas and Oceania. The ecogeographical conditions such as climate and soil, genetic drift and mating system could be the crucial factors for genetic divergence. Furthermore, the study also indicated that northern Africa, Europe and temperate Asia might be the diversity differentiation center of orchardgrass. The result will facilitate the breeding program and germplasm collection and conservation.