Dynamic alterations in serum IgG N-glycan profiles in the development of colitis-associated colon Cancer in mouse model

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 10; p. 129668
• Main Authors: Gu, Yong, Han, Jing, Liu, Xin, Pan, Yiqing, Xu, Xiaoyan, Sha, Jichen, Ren, Shifang, Gu, Jianxin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2020
• Subjects: animal models; Animals; B-lymphocytes; blood serum; carcinogenesis; carcinogenicity; Colitis - blood; Colitis - complications; Colitis - pathology; Colorectal cancer; colorectal neoplasms; Colorectal Neoplasms - blood; Colorectal Neoplasms - etiology; Colorectal Neoplasms - pathology; dextran sulfate; Disease Models, Animal; Dynamic; Female; fucose; galactose; genetic background; Glycomics; Glycosylation; Glycosyltransferase; glycosyltransferases; Immunoglobulin G; Immunoglobulin G - analysis; Immunoglobulin G - blood; Mice; N-glycan; Polysaccharides - analysis; Polysaccharides - blood; sialic acid; ultra-performance liquid chromatography; Dynamic; N-glycan; Glycosyltransferase; Immunoglobulin G; Colorectal cancer
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2020.129668

Alternative glycosylation of serum IgG has been shown to be closely associated with colorectal cancer (CRC). Currently, a dynamic study which can not only minimize the influence of genetic background, environment and other interfering factors during cancer development, but also focus on investigating carcinogenic characteristics of IgG glycan is lacking. Serum IgG N-glycans were characterized at four stages of CRC development by ultra-performance liquid chromatography in a typical colitis-related CRC mouse model induced by azoxymethane-dextran sodium sulfate. Furthermore, the expression of related glycosyltransferases in splenic B lymphocytes at the corresponding time was also assessed. The relative abundance of seven IgG glycans, which can be classified as monoantennary, core fucose, sialic acid, galactose and bisecting, was changed during tumor growth. The abundance of some glycans was altered during the first stage of cancer induction. Correspondingly, the expression of glycosyltransferases in splenic B lymphocytes and different tissues in cancer groups was also decreased compared to that in controls. This study represents the comprehensive analysis of IgG glycosylation in the dynamic process of colitis-associated CRC. To our knowledge, this is the first report that the expression of glycosyltransferases in mouse splenic B lymphocytes is consistent or inconsistent with the alterations of IgG N-glycans, and the variation tendency is tissue nonspecific. Providing a novel approach to identify the IgG glycans related to the development of CRC and laying a foundation for research on structure and function of glycans using mouse. •A dynamic alterations of serum IgG N-glycans with the development of colorectal cancer was uncovered.•The expression of glycosyltransferases in B cells is consistent or inconsistent with the alterations of N-glycans.•The change of glycosyltransferase expression is tissue-specific, but the tendency is nonspecific.