Reagent-less electrogenerated chemiluminescence peptide-based biosensor for the determination of prostate-specific antigen

• Published in: Talanta (Oxford) Vol. 100; pp. 162 - 167
• Main Authors: Qi, Honglan, Wang, Chen, Qiu, Xiaoying, Gao, Qiang, Zhang, Chengxiao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.10.2012; Elsevier
• Subjects: Amino Acid Sequence; Analytical chemistry; Biological and medical sciences; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Chemistry; Electrochemical methods; Electrochemistry; Electrogenerated chemiluminescence; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Humans; Immobilized Proteins - chemistry; Immobilized Proteins - metabolism; Interactions. Associations; Intermolecular phenomena; Kinetics; Limit of Detection; Luminescent Measurements - methods; Methods. Procedures. Technologies; Models, Molecular; Molecular biophysics; Molecular Conformation; Oligopeptides - chemistry; Oligopeptides - metabolism; Peptide; Prostate-specific antigen; Prostate-Specific Antigen - analysis; Prostate-Specific Antigen - metabolism; Various methods and equipments; Prostate-specific antigen; Peptide; Biosensor; Electrogenerated chemiluminescence; Self assembly; Cysteine; Peptides; Probe; Prostate specific antigen; Ester; Target; Signal; Proteolysis; Detection limit; Cleavage; Dicarboxylic acid; Gold; Thiol; Ruthenium; Use; Bipyridines; Concentration; Electrochemiluminescence; Method; Chemical sensor; Potentiometry; Protein; Lysine; Reagents; Molecular recognition; End; Transduction; Technique
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.08.037

A novel electrogenerated chemiluminescence peptide-based (ECL-PB) biosensor for highly sensitive and selective determination of prostate-specific antigen (PSA) was developed. A helix peptide (CHSSKLQK) was served as a molecular recognition element and ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-N-hydroxysuccinimide ester (Ru(bpy)2(dcbpy)NHS) was used as an ECL label. The helix peptide was labeled with the ECL label at NH2-containing lysine and utilized as ECL probe (Ru-peptide). The ECL-PB biosensor was fabricated by immobilizing the ECL probe onto a gold electrode surface via self-assembling technique through a thiol-containing cysteine at the end of the peptide. The principle of ECL measurement is based on the specific proteolytic cleavage event of Ru-peptide on the gold electrode surface in the presence of PSA, resulting in a decrease of ECL signal. The decreased ECL intensity was directly linear to the concentration of PSA in the range from 1.0×10−10g/mL to 8.0×10−9g/mL with a detection limit of 3.8×10−11g/mL. This work demonstrates that the direct transduction of peptide cleavage events into an ECL signal provides a simple and sensitive method for detecting target protein. ► An ECL peptide-based biosensor for prostate-specific antigen was fabricated. ► A helix peptide was employed as molecular recognition element. ► The detection limit for PSA was 0.038ng/mL. ► The kinetic studies of peptide with PSA were performed. ► The method provides a promising strategy for detecting protein with simplicity.