Mangiferin corrects the imbalance of Th17/Treg cells in mice with TNBS-induced colitis

• Published in: International immunopharmacology Vol. 34; pp. 220 - 228
• Main Authors: Lim, Su-Min, Jeong, Jin-Ju, Choi, Hyun Sik, Chang, Hwan Bong, Kim, Dong-Hyun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2016
• Subjects: Anemarrhena - immunology; Animals; Cell Differentiation - drug effects; Cells, Cultured; Colitis; Colitis - chemically induced; Colitis - drug therapy; Colitis - immunology; Drugs, Chinese Herbal - therapeutic use; Forkhead Transcription Factors - metabolism; Homeostasis; Humans; Interleukin-10 - genetics; Interleukin-10 - metabolism; Interleukin-17 - genetics; Interleukin-17 - metabolism; Macrophage; Male; Mangiferin; Mice; Mice, Inbred C57BL; NF-kappa B - metabolism; Nuclear Receptor Subfamily 1, Group F, Member 3 - genetics; Nuclear Receptor Subfamily 1, Group F, Member 3 - metabolism; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; Th17 cell; Th17 Cells - drug effects; Th17 Cells - immunology; Treg cell; Trinitrobenzenesulfonic Acid; Xanthones - therapeutic use; Colitis; Th17 cell; Treg cell; Mangiferin; Macrophage
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2016.03.004

In the previous study, 80% ethanol extract of the rhizome mixture of Anemarrhena asphodeloides and Coptidis chinensis (AC) and its main constituent mangiferin improved TNBS-induced colitis in mice by inhibiting macrophage activation related to the innate immunity. In the preliminary study, we found that AC could inhibit Th17 cell differentiation in mice with TNBS-induced colitis. Therefore, we investigated whether AC and it main constituent mangiferin are capable of inhibiting inflammation by regulating T cell differentiation related to the adaptive immunity in vitro and in vivo. AC and mangiferin potently suppressed colon shortening and myeloperoxidase activity in mice with TNBS-induced colitis. They also suppressed TNBS-induced Th17 cell differentiation and IL-17 expression, but increased TNBS-suppressed Treg cell differentiation and IL-10 expression. Moreover, AC and mangiferin strongly inhibited the expression of TNF-α and IL-17, as well as the activation of NF-κB. Furthermore, mangiferin potently inhibited the differentiation of splenocytes into Th7 cells and increased the differentiation into Treg cells in vitro. Mangiferin also inhibited RORγt and IL-17 expression and STAT3 activation in splenocytes and induced Foxp3 and IL-10 expression and STAT5 activation. Based on these findings, mangiferin may ameliorate colitis by the restoration of disturbed Th17/Treg cells and inhibition of macrophage activation. [Display omitted] •Mangiferin inhibited the differentiation of splenic T cells into Th17 cells in vitro.•Mangiferin suppressed TNBS-induced colitis in mice.•Mangiferin inhibited TNBS-induced RORγt and IL-17 expression and Th17 cell differentiation.•Mangiferin increased TNBS-suppressed Foxp3 and IL-10 expression and Treg differentiation.