Characteristic glycopeptides associated with extreme human longevity identified through plasma glycoproteomics

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 6; pp. 1462 - 1471
• Main Authors: Miura, Yuri, Hashii, Noritaka, Ohta, Yuki, Itakura, Yoko, Tsumoto, Hiroki, Suzuki, Junya, Takakura, Daisuke, Abe, Yukiko, Arai, Yasumichi, Toyoda, Masashi, Kawasaki, Nana, Hirose, Nobuyoshi, Endo, Tamao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2018
• Subjects: Adult; Age Factors; Aged; Aged, 80 and over; biomarkers; Biomarkers - blood; Blood Proteins - metabolism; Case-Control Studies; discriminant analysis; Erythrina; Female; glycopeptides; Glycopeptides - blood; Glycoproteins - blood; glycoproteomics; Glycosylation; haptoglobins; hepatoma; Humans; LC/MS; Lectin microarray; lectins; liquid chromatography; liver cirrhosis; Longevity; Longevity - physiology; mass spectrometry; microarray technology; N-glycosylation; Phaseolus vulgaris; polysaccharides; Polysaccharides - blood; Proteomics - methods; Semi-supercentenarian; Young Adult; CRP; ECA; Lectin microarray; PHAL; OPLS-DA; HCC; GPT; Longevity; GOT; N-glycosylation; IL-6; LC/MS; RBC; TNF-α; ANOVA; SSCs; ALB; LC; PLT; WBC; HB; Semi-supercentenarian; TP; γGTP
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2018.03.025

Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity. Semi-supercentenarians (SSCs; older than 105 years) are thought to be a model of human longevity. Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity. Plasma proteins from Japanese semi-supercentenarians (SSCs, 106–109 years), aged controls (70–88 years), and young controls (20–38 years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS). Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA). Furthermore, plasma levels of clinical biomarkers were measured. We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs. Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs. Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis. However, the SSCs analysed here did not suffer from these diseases. Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity. We found abundant glycans in SSCs, which may be associated with human longevity. •Glycoproteomics was performed using plasma of semi-supercentenarians (SSCs).•Tri-antennary and sialylated N-glycans on haptoglobin were abundant in SSCs.•Clinical biomarkers indicated the subjects of SSCs as healthy.•These characteristic N-glycans in SSCs might be associated with human longevity.