Recombinant IL-2 therapy reverses diminished granulomatous responsiveness in anti-L3T4-treated, Schistosoma mansoni-infected mice

• Published in: The Journal of immunology (1950) Vol. 144; no. 11; pp. 4356 - 4361
• Main Authors: Mathew, RC, Ragheb, S, Boros, DL
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.06.1990; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Monoclonal - therapeutic use; Biological and medical sciences; CD4 Antigens - immunology; CD4-Positive T-Lymphocytes - immunology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Granuloma - pathology; Granuloma - prevention & control; Immunobiology; Immunotherapy; Interleukin-2 - biosynthesis; Interleukin-2 - therapeutic use; Lymphokines, interleukins ( function, expression); Mice; Mice, Inbred Strains; Recombinant Proteins; Regulatory factors and their cellular receptors; Schistosomiasis mansoni - pathology; Schistosomiasis mansoni - physiopathology; Schistosomiasis mansoni - therapy; Spleen - physiopathology; T-Lymphocytes, Regulatory - immunology; Time Factors; Schistosoma mansoni; Rodentia; Cytokine; Plathelmintha; Granulomatosis; Immune regulation; Trematoda; Vertebrata; Mammalia; Interleukin 2; Mouse; Helmintha; Recombinant protein; Invertebrata
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.144.11.4356

We have previously shown, that anti-L3T4 mAb treatment strongly suppressed granuloma formation in the liver, and IL-2 production in the spleen of Schistosoma mansoni-infected mice. In the present study the dynamics of IL-2 production was delineated during the infection, and the effect of rIL-2 treatment on granulomatous responsiveness was examined. IL-2 production in soluble egg Ag-stimulated spleen cells of mice was detectable at 6, peaked at 8 and waned by 20 wk of the infection. In contrast, Con A stimulus elicited high levels of IL-2 production by 8 wk which remained nearly unchanged throughout the infection. Administration of rIL-2 to acutely infected, anti-L3T4 mAb-treated, or chronically infected mice reversed the diminished or modulated granulomatous responses without restoring the ability for endogenous IL-2 production. Transfer of spleen cells of anti-L3T4 mAb-treated, chronically infected mice did not indicate a role for Ts cells in the impaired production of IL-2 in recipients. These data suggest that lack of IL-2 production can play an important role in the immunoregulation of the granulomatous response.