The physiology of salinity tolerance in larvae of two species of Culex mosquitoes: the role of compatible solutes

• Published in: Journal of experimental biology Vol. 203; no. Pt 4; pp. 821 - 830
• Main Authors: Patrick, M L, Bradley, T J
• Format: Journal Article
• Language: English
• Published: England 01.02.2000
• Subjects: Adaptation, Physiological; Amino Acids - blood; Animals; Culex - growth & development; Culex - physiology; Culex quinquefasciatus; Culex tarsalis; Culicidae; Drinking; Fresh Water; Hemolymph - chemistry; Larva - physiology; Osmolar Concentration; Seawater; Sodium - blood; Sodium Chloride; Species Specificity
• ISSN: 0022-0949; 1477-9145
• DOI: 10.1242/jeb.203.4.821

We investigated the physiological basis for differences in salinity tolerance ranges in mosquito larvae of the genus Culex. We examined the response of larvae of C. quinquefasciatus, a freshwater obligate, and C. tarsalis, a euryhaline osmoconformer, following transfer from fresh water to 34% sea water. Hemolymph Na(+) and Cl(-) levels increased similarly in both species, indicating that ion regulation does not differ under these conditions. C. quinquefasciatus responded to increased environmental salinity with increased hemolymph levels of serine, but suffered a significant reduction in levels of trehalose. C. tarsalis responded to increased environmental salinity with increased hemolymph levels of both proline and trehalose. When C. tarsalis larvae were held in 64% sea water, which C. quinquefasciatus larvae cannot tolerate, hemolymph proline and trehalose were accumulated approximately 50-fold and twofold, respectively, relative to freshwater values. We found that proline serves as both an intra- and extracellular compatible solute in C. tarsalis, the first such circumstance documented in an animal in response to increased environmental salinity. Analyses of the acute responses of the two species to an increase in salinity (from 30% to 50% sea water) indicate that larvae of C. tarsalis are able to volume-regulate via drinking and to attenuate increases in hemolymph NaCl concentration using unknown mechanisms during large, rapid increases in salinity.