Candida albicans and Pseudomonas aeruginosa Interact To Enhance Virulence of Mucosal Infection in Transparent Zebrafish
Polymicrobial infections often include both fungi and bacteria and can complicate patient treatment and resolution of infection. Cross-kingdom interactions among bacteria, fungi, and/or the immune system during infection can enhance or block virulence mechanisms and influence disease progression. Th...
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Published in | Infection and immunity Vol. 85; no. 11 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.11.2017
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Subjects | |
Online Access | Get full text |
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Summary: | Polymicrobial infections often include both fungi and bacteria and can complicate patient treatment and resolution of infection. Cross-kingdom interactions among bacteria, fungi, and/or the immune system during infection can enhance or block virulence mechanisms and influence disease progression. The fungus
and the bacterium
are coisolated in the context of polymicrobial infection at a variety of sites throughout the body, including mucosal tissues such as the lung.
,
and
have a bidirectional and largely antagonistic relationship. Their interactions
remain poorly understood, specifically regarding host responses in mediating infection. In this study, we examine trikingdom interactions using a transparent juvenile zebrafish to model mucosal lung infection and show that
and
are synergistically virulent. We find that high
burden, fungal epithelial invasion, swimbladder edema, and epithelial extrusion events serve as predictive factors for mortality in our infection model. Longitudinal analyses of fungal, bacterial, and immune dynamics during coinfection suggest that enhanced morbidity is associated with exacerbated
pathogenesis and elevated inflammation. The
quorum-sensing-deficient Δ
mutant also enhances
pathogenicity in coinfection and induces extrusion of the swimbladder. Together, these observations suggest that
cross talk
can benefit both organisms to the detriment of the host. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/iai.00475-17 |