The unusual X-form DNA in oligodeoxynucleotides: dependence of stability on the base sequence and length

• Published in: Journal of biomolecular structure & dynamics Vol. 13; no. 6; p. 999
• Main Authors: Kypr, J, Chládková, J, Arnold, L, Sági, J, Szemzö, A, Vorlícková, M
• Format: Journal Article
• Language: English
• Published: England 01.06.1996
• Subjects: Base Sequence; Circular Dichroism; DNA - chemistry; Models, Molecular; Nucleic Acid Conformation; Oligodeoxyribonucleotides - chemistry; Purines - chemistry; Pyrimidines - chemistry; Structure-Activity Relationship; Temperature

X-form is an unusual double helix of DNA adopted by poly(dA-dT) or (dT-dA)4 at high concentrations of CsF. On the other hand, poly(dA), poly(dT), (dA-dT)4 and most other DNAs do not adopt this conformer. Here we demonstrate that the X-form is strongly destabilized by GC pairs or even minute perturba...