Cellulase-assisted extraction and antioxidant activity of the polysaccharides from garlic

• Published in: Carbohydrate polymers Vol. 111; pp. 606 - 609
• Main Authors: Pan, Saikun, Wu, Shengjun
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 13.10.2014; Elsevier
• Subjects: Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - metabolism; Antioxidants - pharmacology; Applied sciences; Biological and medical sciences; Cellulase - metabolism; Cellulase-assisted extraction; Exact sciences and technology; Garlic - chemistry; General and cellular metabolism. Vitamins; Hydroxyl Radical - chemistry; Medical sciences; Natural polymers; Pharmacology. Drug treatments; Physicochemistry of polymers; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - metabolism; Plant Extracts - pharmacology; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - metabolism; Polysaccharides - pharmacology; Starch and polysaccharides; Polysaccharides; Cellulase-assisted extraction; Antioxidant activity; Monocotyledones; Solvent extraction; Optimization; Radical trapping; Scavenging efficiency; Angiospermae; Hydroxyl radicals; Allium sativum; DPPH; Enzyme; Antioxidant; Experimental study; Operating conditions; Glycosylases; Enzymatic method; Liliaceae; Cellulase; Glycosidases; Plant origin; Hydrolases; Oside polymer; Spermatophyta; Garlic
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2014.05.022

•The extraction conditions were 80min, 45°C, pH 5, cellulase 8000U/g.•The GPS yield reached up to 35.34%.•The GPS showed strong antioxidant ability. In the present study, the polysaccharides were prepared from garlic by using a cellulase-assisted extraction method and the antioxidant activity of garlic polysaccharides (GPs) was evaluated. To improve the yield of GPs, the influences of the several factors such as extraction time, temperature, pH, and cellulase amount on the extraction efficiency were studied. The optimal conditions for extraction of GPs were determined as follows: time, 80min; temperature, 45°C; pH, 5; cellulase amount, 8000U/g. Under the optimised extraction conditions, the yield of GPS reached up to 35.34%. The GPs product exhibited strong antioxidant activity including hydroxyl radical scavenging activity, 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity, and reducing power. The results suggest that the GPs could be used as potential antioxidants.