Assignment of Human Glutaryl-CoA Dehydrogenase Gene (GCDH) to the Short Arm of Chromosome 19 (19p13.2) by in Situ Hybridization and Somatic Cell Hybrid Analysis

• Published in: Genomics (San Diego, Calif.) Vol. 21; no. 1; pp. 289 - 290
• Main Authors: Greenberg, Cheryl R., Duncan, Alessandra M.V., Gregory, Carolyn A., Singal, Rupinder, Goodman, Stephen I.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.1994; Elsevier
• Subjects: Animals; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOTECHNOLOGY; Chromosome Mapping; CHROMOSOMES; Chromosomes, Human, Pair 19; Classical genetics, quantitative genetics, hybrids; Cricetinae; DNA HYBRIDIZATION; ENZYMES; Fundamental and applied biological sciences. Psychology; GENES; GENETIC ENGINEERING; GENETIC MAPPING; Genetics of eukaryotes. Biological and molecular evolution; Glutaryl-CoA Dehydrogenase; Human; HUMAN CHROMOSOME 19; HUMAN CHROMOSOMES; Humans; Hybrid Cells; HYBRIDIZATION; In Situ Hybridization; MAPPING; NUCLEIC ACID HYBRIDIZATION; ORGANIC COMPOUNDS; OXIDOREDUCTASES; Oxidoreductases - genetics; Oxidoreductases Acting on CH-CH Group Donors; PROTEINS 550400 > Genetics; Genetic mapping; Human; Gene; Enzyme; Glutaryl-CoA dehydrogenase; Oxidoreductases; F19-Chromosome
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1994.1264

Here, the authors report the mapping of GCDH to chromosome 19 by both in situ hybridization and human-hamster somatic cell hybrid analysis. In situ hybridization of a 688-bp genomic fragment of GCDH to BrdU-synchronized peripheral blood lymphocytes was performed as described in Duncan et al. The positions of silver grains directly over or touching well-banded metaphase chromosomes were mapped to an ISCN idiogram. The same 688-bp genomic fragment was hybridized to a Southern blot of the same panel of EcoRI-digested DNA from 22 hybrid cell lines containing various complements of human and hamster chromosomes, one hamster cell line, and parental human lymphoblasts as previously described. The GCDH gene segregated with human chromosome 19 with 100% concordance for the absence of presence of chromosome 19 (with positive isozymes for chromosome 19) (data not shown). The analysis of the distribution of 600 grains following in situ hybridization revealed a significant clustering of grains in the short arm of chromosome 19. Of 600 grains, 94 mapped to this region, with a peak distribution at 19p13.2. 8 refs., 1 fig.