Model based analysis of transient fluorescence yield induced by actinic laser flashes in spinach leaves and cells of green alga Chlorella pyrenoidosa Chick

• Published in: Plant physiology and biochemistry Vol. 77; pp. 49 - 59
• Main Authors: Belyaeva, N.E., Schmitt, F.-J., Paschenko, V.Z., Riznichenko, G. Yu, Rubin, A.B., Renger, G.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.04.2014
• Subjects: Chlorella - chemistry; Chlorella - metabolism; Chlorella pyrenoidosa; Chlorophyll - metabolism; Electron transfer; Electron Transport; Fluorescence; Fluorescence yield; Lasers; Light; Model simulation; Models, Biological; Oxidation-Reduction; Photosynthesis; Photosystem II; Photosystem II Protein Complex - metabolism; Plant Leaves - chemistry; Plant Leaves - metabolism; Proton transfer; Protons; Single turnover flash; Spectrometry, Fluorescence; Spinacia oleracea; Spinacia oleracea - chemistry; Spinacia oleracea - metabolism; Water - chemistry; Water oxidizing complex; Fluorescence yield; Proton transfer; Single turnover flash; Electron transfer; Water oxidizing complex; Photosystem II; Model simulation
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2014.01.017

Measurements of Single Flash Induced Transient Fluorescence Yield (SFITFY) on spinach leaves and whole cells of green thermophilic alga Chlorella pyrenoidosa Chick were analyzed for electron transfer (ET) steps and coupled proton transfer (PT) on both the donor and the acceptor side of the reaction center (RC) of photosystem II (PS II). A specially developed PS II model (Belyaeva et al., 2008, 2011a) allowed the determination of ET steps that occur in a hierarchically ordered time scale from nanoseconds to several seconds. Our study demonstrates that our SFITFY data is consistent with the concept of the reduction of P680+ by YZ in both leaves and algae (studied on spinach leaves and cells of Chlorella pyrenoidosa Chick). The multiphasic P680+ reduction kinetics by YZ in PS II core complexes with high oxygen evolution capacity was seen in both algae and leaves. Model simulation to fit SFITFY curves for dark adapted species used here gives the rate constants to verify nanosecond kinetic stages of P680+ reduction by YZ in the redox state S1 of the water oxidizing complex (WOC) shown in Kühn et al. (2004). Then a sequence of relaxation steps in the redox state S1, outlined by Renger (2012), occurs in both algae and leaves as a similar non-adiabatic ET reactions. Coupled PT is discussed briefly to understand a rearrangement of hydrogen bond protons in the protein matrix of the WOC (Umena et al., 2011). On the other hand, present studies showed a slower reoxidation of reduced QA by QB in algal cells as compared with that in a leaf that might be regarded as a consequence of differences of spatial domains at the QB-site in leaves compared to algae. Our comparative study helped to correlate theory with experimental data for molecular photosynthetic mechanisms in thylakoid membranes. •10 ns saturating Flash Induces Fluorescence Yield, SFITFY, on dark adapted samples.•SFITFY patterns in 100 ns-10 s reflect photosystem II (PSII) electron transfer (ET).•PS II model simulates donor and acceptor side ET to fit algal or leaf SFITFY.•Redox state S1 relaxation steps show similar algal and leaf multiphasic kinetics.•Thermophilic Chlorella shows ET from reduced QA to QB slower than in spinach leaf.