A comparison study on extraction of polysaccharides from Tricholoma matsutake by response surface methodology

• Published in: Carbohydrate polymers Vol. 102; pp. 419 - 422
• Main Authors: Yin, Xiulian, You, Qinghong, Su, Xiaoye
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.02.2014; Elsevier
• Subjects: Applied sciences; Biological and medical sciences; Counter-current probe ultrasonic extraction; Energy consumption; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Microbiology; Morphology, structure, chemical composition; Mycology; Natural polymers; Physicochemistry of polymers; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Response surface methodology; Starch and polysaccharides; Surface Properties; Tricholoma - chemistry; Tricholoma matsutake polysaccharides; Counter-current probe ultrasonic extraction; Energy consumption; Response surface methodology; Tricholoma matsutake polysaccharides; Experimental study; Solvent extraction; Optimization; Basidiomycota; Operating conditions; Fungi; Sporocarp; Oside polymer; Ultrasound assisted process; Edible fungi
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.11.072

•CCPUE method for the extraction of Tricholoma matsutake's polysaccharides was built.•HWE, UCE, EAE, SUPE and CCPUE were compared for extracting TMP.•The TMP yield of CCPUE was higher than that of other methods.•The energy consumption of CCPUE was modest. The Tricholoma matsutake polysaccharides (TMP) extraction was optimized using counter-current probe ultrasonic extraction (CCPUE) by response surface methodology (RSM). The effect of three factors (liquid–solid ratio, extraction time and pulse duration) on the TMP yield was studied. The results showed that the optimal conditions were liquid–solid ratio (15), extraction time (61min) and pulse duration (6.0s). Under best conditions, the maximum TMP yield was 8.11±0.35%, which was close with the predicted yield value (8.03%). The five methods, hot-water extraction (HWE), enzyme assisted extraction (EAE), ultrasonic clearer extraction (UCE), static probe ultrasonic extraction (SPUE), and CCPUE for extracting TMP by RSM were further compared. The results indicated that CCPUE had the largest yield of TMP with modest energy consumption.