Protective effect of delta opioid agonist [d-Ala2, d-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits

• Published in: Neuroscience letters Vol. 584; pp. 1 - 6
• Main Authors: Liu, Haitong, Chen, Binbin, Zhang, Yi, Qiu, Yimin, Xia, Yunfei, Li, Shitong, Yao, Junyan
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.01.2015
• Subjects: Animals; Aorta, Abdominal; DADLE; Enkephalin, Leucine-2-Alanine - administration & dosage; Enkephalin, Leucine-2-Alanine - therapeutic use; Female; Hemodynamics - drug effects; Ischemia reperfusion injury; Male; Neurons - drug effects; Neurons - pathology; Neuroprotection; Neuroprotective Agents - administration & dosage; Neuroprotective Agents - therapeutic use; Perfusion; Rabbits; Receptors, Opioid, delta - agonists; Reperfusion Injury - pathology; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Spinal cord; Spinal Cord Ischemia - pathology; Spinal Cord Ischemia - physiopathology; Spinal Cord Ischemia - prevention & control; Neuroprotection; Rabbits; Spinal cord; Ischemia reperfusion injury; DADLE
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2014.09.048

•DADLE is beneficial to spinal cord ischemia-reperfusion injury.•DADLE regional perfusion through abdominal aorta may possess neuroprotective efficacy.•DADLE improved the neurological dysfunction.•DADLE also reduced the neuronal injury. [d-Ala2, d-Leu5] enkephalin (DADLE) has been reported to exhibit protective effects against hypoxic or ischemic induced brain insult. However its efficacy on the spinal cord ischemia-reperfusion injury remains unclear. Here we investigate whether DADLE could attenuate ischemia and reperfusion induced neural injury in the rabbit spinal cord. New Zealand white rabbits were subjected to spinal cord ischemia by infrarenal aortic occlusion for 30min. In the period of spinal cord ischemia, DADLE 0.5mg/kg or NS were infused continuously into the distal clamped abdominal aorta. The heart rate, blood pressure, and core temperature were monitored continuously during the whole experimental procedure. Then the neurological behavioral function was assessed with Tarlov scale system at 1h, 6h, 24h, 48h after reperfusion, and neuronal injury evaluation in the ventral horn of gray matter was measured by counting the normal motor neurons at 48h after reperfusion. Comparing with the control group, the Tarlov scores were significantly higher and the incidences of paraplegia were significantly lower in the DADLE group at four time-point recorded. In addition, the normal neurons numbers in the DADLE group were significant more than those in the control group at 48h after reperfusion. These results suggested that DADLE infused into the abdominal aorta during ischemia period could attenuate behavioral retardation and the loss of normal motor neuron induced by ischemia-reperfusion in rabbits.