Lethal and sublethal effects of the chitin synthesis inhibitor chlorfluazuron on Bradysia odoriphaga Yang and Zhang (Diptera: Sciaridae)

• Published in: Pesticide biochemistry and physiology Vol. 136; pp. 80 - 88
• Main Authors: Zhang, Peng, Zhao, Yun-He, Wang, Qiu-Hong, Mu, Wei, Liu, Feng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2017
• Subjects: Animals; bioassays; Bradysia odoriphaga; Bradysia odoriphaga Yang and Zhang; China; chitin; Chitin - antagonists & inhibitors; Chitin - biosynthesis; Chlorfluazuron; Diptera - drug effects; Diptera - metabolism; Diptera - physiology; fecundity; Female; Glutathione S-transferase; glutathione transferase; Glutathione Transferase - metabolism; ingestion; insect pests; Insect Proteins - metabolism; Insecticides - toxicity; instars; integrated pest management; Larva - drug effects; Larva - metabolism; Larva - physiology; larvae; larval development; lethal concentration 50; Male; Microsomal mixed function oxidase; mixed function oxidase; Mixed Function Oxygenases - metabolism; Phenylurea Compounds - toxicity; Population dynamics; population growth; Pyridines - toxicity; Reproduction - drug effects; sublethal effects; survival rate; Two-sex life table; China; Population dynamics; Bradysia odoriphaga Yang and Zhang; Chlorfluazuron; Glutathione S-transferase; Microsomal mixed function oxidase; Two-sex life table
• ISSN: 0048-3575; 1095-9939; 1095-9939
• DOI: 10.1016/j.pestbp.2016.07.007

Bradysia odoriphaga Yang and Zhang is the primary insect pest that affects Chinese chive in northern China. Nevertheless, very few studies have been conducted on the use of chitin synthesis inhibitors (CSIs) for the control of B. odoriphaga. Here, lethal and sublethal effects of the CSI chlorfluazuron on B. odoriphaga were studied to explore the use for integrated pest management (IPM) of B. odoriphaga. A contact and ingestion toxicity bioassay showed that chlorfluazuron was more active against B. odoriphaga than three other CSIs, with a 72h LC50 of 0.1593mg/L. Treatment with the LC50 dose of chlorfluazuron decreased both the intrinsic and finite rates of increase of B. odoriphaga, in addition to reproduction rate, survival rate, and fecundity, and the mean generation time, total preovipositional period and larval development duration were shortened, compared with those of the control and LC10 groups. The mean generation time, total preovipositional period and larval development duration were all also markedly decreased by treatment with chlorfluazuron at the LC10. Furthermore, chlorfluazuron inhibited the feeding of the final instar larvae for a short period. Glutathione S-transferase and microsomal mixed function oxidase activities increased after exposure to the chemical. These results showed that chlorfluazuron at the sublethal LC50 treatment inhibited B. odoriphaga population growth, whereas the danger of causing rapid population growth by using a lower sublethal concentration was demonstrated with the sublethal LC10 treatment. Therefore, chlorfluazuron should be used with caution in an IPM program for B. odoriphaga. [Display omitted] •Chlorfluazuron at low-lethal doses affect growth and development of Bradysia odoriphaga.•LC50 decreased the developmental rate.•LC10 sublethal concentration caused rapid population growth.•Chlorfluazuron enhanced the activities of GST and MFO.•Chlorfluazuron should be used with caution in IPM of B. odoriphaga.