Detergents and alternatives in cryo-EM studies of membrane proteins

Structure determination of membrane proteins has been a long-standing challenge to understand the molecular basis of life processes. Detergents are widely used to study the structure and function of membrane proteins by various experimental methods, and the application of membrane mimetics is also a...

Full description

Saved in:
Bibliographic Details
Published inActa biochimica et biophysica Sinica Vol. 54; no. 8; pp. 1049 - 1056
Main Author Li, Shuo
Format Journal Article
LanguageEnglish
Published China China Science Publishing & Media Ltd 25.08.2022
Subjects
cryo-EM
Cryoelectron Microscopy - methods
detergent
Detergents - chemistry
Detergents - metabolism
membrane protein
Membrane Proteins - metabolism
micelle
nanodiscs
detergent
micelle
membrane protein
nanodiscs
cryo-EM
Online AccessGet full text

Cover

More Information
Summary:Structure determination of membrane proteins has been a long-standing challenge to understand the molecular basis of life processes. Detergents are widely used to study the structure and function of membrane proteins by various experimental methods, and the application of membrane mimetics is also a prevalent trend in the field of cryo-EM analysis. This review focuses on the widely-used detergents and corresponding properties and structures, and also discusses the growing interests in membrane mimetic systems used in cryo-EM studies, providing insights into the role of detergent alternatives in structure determination.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Review-3
content type line 23
ISSN:1672-9145
1745-7270
1745-7270
DOI:10.3724/abbs.2022088