Antibody repertoire sequencing analysis

• Published in: Acta biochimica et biophysica Sinica Vol. 54; no. 6; pp. 864 - 873
• Main Authors: Zhang, Senxin, Yang, Tiange, Liu, Xiaojing, Yang, Jiyuan, Zheng, Xiaoqi
• Format: Journal Article
• Language: English
• Published: China China Science Publishing & Media Ltd 01.06.2022
• Subjects: BCR repertoire analysis; Cells, Cultured; High-Throughput Nucleotide Sequencing - methods; high-throughput sequencing; Phylogeny; profiling and data mining; Receptors, Antigen, B-Cell - genetics; statistical method; BCR repertoire analysis; profiling and data mining; statistical method; high-throughput sequencing
• ISSN: 1672-9145; 1745-7270
• DOI: 10.3724/abbs.2022062

High-throughput sequencing for B cell receptor (BCR) repertoire provides useful insights for the adaptive immune system. With the continuous development of the BCR-seq technology, many efforts have been made to develop methods for analyzing the ever-increasing BCR repertoire data. In this review, we comprehensively outline different BCR repertoire library preparation protocols and summarize three major steps of BCR-seq data analysis, . ., V(D)J sequence annotation, clonal phylogenetic inference, and BCR repertoire profiling and mining. Different from other reviews in this field, we emphasize background intuition and the statistical principle of each method to help biologists better understand it. Finally, we discuss data mining problems for BCR-seq data and with a highlight on recently emerging multiple-sample analysis.