Starch slurry hydrolysis using α-amylase immobilized on a hollow-fiber reactor

• Published in: Enzyme and microbial technology Vol. 17; no. 8; pp. 685 - 688
• Main Authors: Ju, Yi-Hsu, Chen, Wen-Jen, Lee, Cheng-Kang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 1995; Elsevier Science
• Subjects: Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; hollow fibers; immobilization; Methods. Procedures. Technologies; Starch hydrolysis; α-amylase; α-amylase; immobilization; hollow fibers; Starch hydrolysis; Viscosity; Enzyme; Starch; Continuous process; Hydrolysis; Bioreactor; Batchwise; Glycosidases; Hollow fiber; Hydrolases; α-Amylase; O-Glycosidases; Immobilized enzyme
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/0141-0229(94)00034-O

A hollow-fiber hemodialyzer with α-amylase immobilized on the lumen and outer surface of hollow fibers was employed to hydrolyze the high-concentration starch slurry. The viscous starch slurry was first hydrolyzed in the shell side of the hollow-fiber reactor, and the unconverted starch was further hydrolyzed as the slurry circulated through the narrow lumen of hollow fibers. The reactor retained 65% of its initial activity after a 30-batch reaction at 65°C. Starch slurry of 30% (wt/vol) can be hydrolyzed without any difficulties by the hollow-fiber reactor. In continuous operation, a starch hydrolysate of dextrose equivalent 12% was obtained at mean residence time of 9.5 min.