Minimal medium recovery of heated Salmonella typhimurium LT2

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, U.S.A. ABSTRACT Summary: Exponential phase Salmonella typhimurium LT2, S. thompson and S. heidelberg grown in a glucose-salts medium (M-9), harvested at 37°C and heat-treated at 50°C exhi...

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Published inJournal of general microbiology Vol. 74; no. 2; pp. 267 - 274
Main Authors Gomez, R.F, Sinskey, A.J, Davies, R, Labuza, T.P
Format Journal Article
LanguageEnglish
Published England Soc General Microbiol 01.02.1973
Subjects
agricultural products
Cell Count
Culture Media
DNA Repair
DNA, Bacterial
Hot Temperature
Hydroxyurea - pharmacology
Salmonella typhimurium - drug effects
Salmonella typhimurium - growth & development
Salmonella typhimurium - metabolism
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Summary:Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, U.S.A. ABSTRACT Summary: Exponential phase Salmonella typhimurium LT2, S. thompson and S. heidelberg grown in a glucose-salts medium (M-9), harvested at 37°C and heat-treated at 50°C exhibit a higher recovery on M-9 agar than on Trypticase Soy agar with yeast extract. This phenomenon is analogous to minimal medium recovery of irradiated bacteria. Supplementation of M-9 agar with yeast extract or Casamino acids resulted in lower viable counts of heat-treated bacteria than on M-9 agar alone. Heat-treated bacteria incubated at 37°C in M-9 broth or distilled water recovered their ability to grow on TSY agar. This recovery is inhibited by hydroxyurea. Present address: National College of Food Technology, University of Reading, St George's Avenue, Weybridge, Surrey, U.K.
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ISSN:0022-1287
DOI:10.1099/00221287-74-2-267