Boosting the denitrification efficiency of iron-based constructed wetlands in-situ via plant biomass-derived biochar: Intensified iron redox cycle and microbial responses

• Published in: Water research (Oxford) Vol. 253; p. 121285
• Main Authors: Fan, Yuanyuan, Sun, Shanshan, Gu, Xushun, Zhang, Manping, Peng, Yuanyuan, Yan, Pan, He, Shengbing
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2024
• Subjects: bioavailability; Biochar; Biomass; bioremediation; Carbon; Charcoal; Constructed wetlands; Denitrification; denitrifying microorganisms; Electron transfer; Fe (III)/Fe (II) cycle; Geobacter; Iris pseudacorus; Iron - chemistry; iron absorption; Nitrate removal; nitrates; Nitrogen; Oxidation-Reduction; Plant biomass; Shewanella; species; wastewater; water; Wetlands; EDC; EAC; Plant biomass; Electron transfer; Biochar; ZVI; Nitrate removal; ETC; Constructed wetlands; MEO; IRB; Fe (III)/Fe (II) cycle; MER; IOB; CWs
• ISSN: 0043-1354; 1879-2448; 1879-2448
• DOI: 10.1016/j.watres.2024.121285

•Biomass and its derived biochar addition enhanced iron scraps bioavailability.•Biochar as electron shuttle sustains a sustainable Fe-N electron transfer.•BC-ICW performed better and longer-lasting TN removal capacity.•Electroactive microbes and autotrophic denitrifiers were enriched in BC-ICW.•Functional genes related to Fe and N metabolism were up-regulated in BC-ICW. Considering the unsatisfied denitrification performance of carbon-limited wastewater in iron-based constructed wetlands (ICWs) caused by low electron transfer efficiency of iron substrates, utilization of plant-based conductive materials in-situ for improving the long-term reactivity of iron substrates was proposed to boost the Fe (III)/Fe (II) redox cycle thus enhance the nitrogen elimination. Here, we investigated the effects of withered Iris Pseudacorus biomass and its derived biochar on nitrogen removal for 165 days in ICWs. Results revealed that accumulate TN removal capacity in biochar-added ICW (BC-ICW) increased by 14.7 % compared to biomass-added ICW (BM-ICW), which was mainly attributed to the synergistic strengthening of iron scraps and biochar. The denitrification efficiency of BM-ICW improved by 11.6 % compared to ICWs, while its removal capacity declined with biomass consumption. Autotrophic and heterotrophic denitrifiers were enriched in BM-ICW and BC-ICW, especially biochar increased the abundance of electroactive species (Geobacter and Shewanella, etc.). An active iron cycle exhibited in BC-ICW, which can be confirmed by the presence of more liable iron minerals on iron scraps surface, the lowest Fe (III)/Fe (II) ratio (0.51), and the improved proportions of iron cycling genes (feoABC, korB, fhuF, TC.FEV.OM, etc.). The nitrate removal efficiency was positively correlated with the nitrogen, iron metabolism functional genes and the electron transfer capacity (ETC) of carbon materials (P < 0.05), indicating that redox-active carbon materials addition improved the iron scraps bioavailability by promoting electron transfer, thus enhancing the autotrophic nitrogen removal. Our findings provided a green perspective to better understand the redox properties of plant-based carbon materials in ICWs for deep bioremediation in-situ. [Display omitted]