Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

• Published in: Journal of Biomedical Optics Vol. 15; no. 5; p. 056016
• Main Authors: Raja, Anju M, Xu, Shuoyu, Sun, Wanxin, Zhou, Jianbiao, Tai, Dean C. S, Chen, Chien-Shing, Rajapakse, Jagath C, So, Peter T. C, Yu, Hanry
• Format: Journal Article
• Language: English
• Published: United States 01.09.2010
• Subjects: Animals; Antineoplastic Agents - therapeutic use; Cell Line, Tumor; Chemotherapy; Collagen - metabolism; Collagens; Drugs; Female; Fibers; Harmonics; Humans; Image Interpretation, Computer-Assisted; Imaging; Indazoles - therapeutic use; Kinases; Lasers; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - metabolism; Leukemia, Myeloid, Acute - pathology; Mice; Mice, SCID; Microscopy - instrumentation; Microscopy - methods; Neoplasms - drug therapy; Neoplasms - metabolism; Neoplasms - pathology; Optical Phenomena; Phenylurea Compounds - therapeutic use; Receptor Protein-Tyrosine Kinases - antagonists & inhibitors; Transplantation, Heterologous; Tumors
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.3497565

Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.