Habitat visualization, acquisition features and necessity of the gammaproteobacterial symbiont of pistachio stink Bug, Acrosternum heegeri (Hem.: Pentatomidae)

• Published in: Bulletin of entomological research Vol. 110; no. 1; pp. 22 - 33
• Main Authors: Kashkouli, M, Fathipour, Y, Mehrabadi, M
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 01.02.2020
• Subjects: Acrosternum heegeri; Animals; Bacteria; Biology; Cell surface; Crypts; Deoxyribonucleic acid; DNA; Eggs; Electron microscopy; Ethanol; Female; Fluorescence; Fluorescence in situ hybridization; Gammaproteobacteria - isolation & purification; Gastrointestinal Tract - microbiology; Gastrointestinal Tract - ultrastructure; Genetic testing; Habitats; Heteroptera - microbiology; Heteroptera - ultrastructure; Hybridization; Insects; Midgut; Nucleotide sequence; Nymphs; Ovary - microbiology; Ovum - microbiology; Pantoea; PCR; Pentatomidae; Pest control; Phylogenetics; Phylogeny; Real time; Scanning electron microscopy; Sterilization; Survivability; Survival; Symbionts; Symbiosis; Transmission electron microscopy; Iran; pentatomidae; symbiont elimination; gammaproteobacteria; Heritable symbiont
• ISSN: 0007-4853; 1475-2670
• DOI: 10.1017/s0007485319000245

Plant-sucking stinkbugs are especially associated with mutualistic gut bacterial symbionts. Here, we explored the symbiotic relationship of a pistachio stinkbug, Acrosternum heegeri Fieber by histological, fluorescence in situ hybridization (FISH), real-time PCR and molecular phylogenetic techniques. Furthermore, the effects of the symbiont on the resting/wandering behaviors of the newborn nymphs, pre-adult survival rates, and stage compositions were investigated. Transmission electron microscopy and real-time PCR analyses showed that a rod-shaped gammaproteobacterium was persistently located within the posterior midgut crypts. Molecular phylogenetic and FISH techniques strongly suggested that this symbiont should be placed in the genus Pantoea of the Enterobacteriales. Scanning electron microscopy confirmed the presence of the bacterial cells on the egg surface which the surface sterilization of the eggs resulted in the successful removal of the symbiont from the eggs. Symbiotic and aposymbiotic A. heegeri showed no significant differences in the wandering behaviors of the first nymphal stages, while the symbiont-free insects suffered retarded growth and lower survivability. Together, the results highlight the habitat and acquisition features of Pantoea symbiont and its contribution in A. heegeri biology that might help us for better pest management in the future.