Pyridoxine improves hippocampal cognitive function via increases of serotonin turnover and tyrosine hydroxylase, and its association with CB1 cannabinoid receptor-interacting protein and the CB1 cannabinoid receptor pathway

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 12; pp. 3142 - 3153
• Main Authors: Jung, Hyo Young, Kim, Dae Won, Nam, Sung Min, Kim, Jong Whi, Chung, Jin Young, Won, Moo-Ho, Seong, Je Kyung, Yoon, Yeo Sung, Yoo, Dae Young, Hwang, In Koo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: Animals; antagonists; cannabinoids; Carrier Proteins - physiology; CB1 cannabinoid receptor-interacting protein 1; cell proliferation; cognition; Cognition - drug effects; Hippocampus; Hippocampus - drug effects; Hippocampus - physiology; Immunohistochemistry; LIM Domain Proteins - physiology; Male; Memory; Mice; Mice, Inbred C57BL; Neurogenesis; Novel object recognition; protein composition; proteomics; Pyridoxine; Pyridoxine - pharmacology; Receptor, Cannabinoid, CB1 - analysis; Receptor, Cannabinoid, CB1 - physiology; serotonin; Serotonin - metabolism; small interfering RNA; tyrosine; tyrosine 3-monooxygenase; Tyrosine 3-Monooxygenase - analysis; Tyrosine 3-Monooxygenase - metabolism; Pyridoxine; Novel object recognition; CB1 cannabinoid receptor-interacting protein 1; Neurogenesis; Hippocampus
• ISSN: 0304-4165; 1872-8006
• DOI: 10.1016/j.bbagen.2017.09.006

In the present study, we investigated the effects of pyridoxine on hippocampal functions and changes in protein profiles based on the proteomic approach. Eight-week-old mice received intraperitoneal injections of physiological saline (vehicle) or 350mg/kg pyridoxine twice a day for 21days. Phosphoglycerate mutase 1 was up-regulated, while CB1 cannabinoid receptor-interacting protein 1 (CRIP1) was down-regulated, in the pyridoxine-treated group. Additionally, the serotonin and tyrosine hydroxylase was increased in the hippocampus of the pyridoxine-treated group than in that of the vehicle-treated group. Furthermore, discrimination indices based on the novel object recognition test were significantly higher in the pyridoxine-treated group than in the vehicle-treated group. Administration of CRIP1a siRNA significantly increases the discrimination index as well as cell proliferation and neuroblast differentiation in the dentate gyrus. In addition, the administration of rimonabant, a CB1 cannabinoid receptor antagonist, for 3weeks significantly decreased the novel object recognition memory, the tyrosine hydroxylase level, the amount of cell proliferation, and neuroblast differentiation in the dentate gyrus. Treatment with pyridoxine significantly increased novel object recognition memory, but slightly ameliorated rimonabant-induced reduction in serotonin, the tyrosine hydroxylase level, the amount of cell proliferation, and neuroblast differentiation in the dentate gyrus. These results suggest that pyridoxine promotes hippocampal functions by increasing serotonin and tyrosine hydroylase immunoreactivity in the hippocampus. This positive effect may be associated with CRIP1a and CB1 cannabinoid receptor function. Vitamin-B6 enhances hippocampal functions and this is closely associated with CRIP1a and CB1 cannabinoid receptors. •Pyridoxine increases novel object recognition memory, serotonin turnover, and tyrosine hydroxylase expression.•Pyridoxine decreases CB1 canabinoid receptor-interacting protein expression.•Knockdown of CB1 cannabinoid receptor-interacting protein increases neurogenesis as well as novel object recognition memory.•Blocking of CB1 cannabinoid receptor inhibits pyridoxine from increasing cell proliferation and neuroblast differentiation.•Pyridoxine increases hippocampal functions by modulating CB1 receptor signalling in the hippocampus.