Postnatal Development of Myosin Heavy Chain Isoforms in Rat Laryngeal Muscles

• Published in: Annals of otology, rhinology & laryngology Vol. 108; no. 5; pp. 509 - 515
• Main Authors: Shiotani, Akihiro, Jones, Richard M., Flint, Paul W.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.05.1999; Annals Publishing Compagny; SAGE PUBLICATIONS, INC
• Subjects: Aging - metabolism; Animals; Biological and medical sciences; Biotechnology; Blotting, Western; Electrophoresis, Polyacrylamide Gel; Ent. Stomatology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; In vitro gene amplification. Pcr technique; Investigative techniques, diagnostic techniques (general aspects); Laryngeal Muscles - chemistry; Medical sciences; Methods. Procedures. Technologies; Myosin Heavy Chains - analysis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Protein Isoforms - analysis; Rats; Rats, Sprague-Dawley; Western blot; myosin heavy chain; development; sodium dodecyl sulfate—polyacrylamide gel electrophoresis; laryngeal muscle; Heavy peptide chain; Rat; Gel electrophoresis; Rodentia; Exploration; Experimental study; Vertebrata; Mammalia; Postnatal; Animal; Blotting assay; Myosin; Development; Genetics; Larynx; Muscle; Molecular biology
• ISSN: 0003-4894; 1943-572X
• DOI: 10.1177/000348949910800517

The developmental transitions of myosin heavy chain (MHC) isoforms of rat posterior cricoarytenoid (PCA), thyroarytenoid (TA), cricothyroid (CT), and lateral cricoarytenoid (LCA) muscles were examined by means of sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot techniques. The muscles were microscopically dissected from animals on postnatal days 0, 3, 7, 10, 14, 21, 28, 35, 45, and 55 and from adult animals. Silver-stained SDS-PAGE gels of each muscle were analyzed densitometrically to measure the composition of MHC isoforms, and Western blot was carried out to identify specific bands. Characterizations of the internal laryngeal muscles determined by the composition of MHCs were correlated with their function in the adult. Temporally, differentiation reflects onset of function. Differentiation of isoforms and transition to adult forms occur first in the TA muscle, followed by the PCA, LCA, and CT muscles. Expression of type IIL was observed only in muscles innervated by the recurrent laryngeal nerve. Postnatally observed developmental differences of myosin phenotypes suggest that regulation of MHC expression is influenced by neural activity or other environmental factors.