A biodegradable in situ injectable hydrogel based on chitosan and oxidized hyaluronic acid for tissue engineering applications

• Published in: Carbohydrate polymers Vol. 85; no. 4; pp. 838 - 844
• Main Authors: Nair, Sandhya, Remya, N.S., Remya, S., Nair, Prabha D.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2011; Elsevier
• Subjects: Applied sciences; Biodegradability; Biological and medical sciences; Carbohydrates; Chitosan; Chondrocytes; encapsulation; Exact sciences and technology; Extracellular matrix; Fibroblasts; freeze drying; gelation; gels; Glycerol; Hyaluronic acid; hydrocolloids; Hydrogels; Medical sciences; Natural polymers; Oxidation; Phosphate; phosphates; Physicochemistry of polymers; Porosity; scaffolds; Starch and polysaccharides; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Tissue engineering; viability; Chitosan; Hydrogels; Hyaluronic acid; Tissue engineering; Biological properties; Encapsulation; Injectable form; Chondrocyte; Biodegradability; Cytotoxicity; Crosslinked copolymer; Colloidal gel; Pore size; Biocompatibility; Biomaterial; Framework; Swelling; Gelation; Crosslinking; Experimental study; In vitro; Cryogel; Chitosan derivatives; Morphology; Preparation; Kinetics; Fibroblast
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.04.004

An “in situ” biodegradable gel consisting of chitosan, glycerol phosphate (GP) and oxidized hyaluronic acid (HDA) were synthesised and characterized .This is a two component hydrogel system where chitosan neutralized with GP resulted in instantaneous gelling when combined with HDA. The gels are cytocompatible and could be freeze dried to form porous scaffolds. The percentage porosity of the freeze-dried chitosan hyaluronic acid dialdehyde gels (CHDA) increased with increasing oxidation. Fibroblast cells seeded onto CHDA porous scaffolds adhered, proliferated and produced ECM components on the scaffold. Chondrocytes encapsulated in CHDA gels retained their viability and specific phenotypic characteristics. The gel material is hence proposed as a scaffold and encapsulating material for tissue engineering applications.