Enterobacter cloacae with a novel variant of ACT AmpC beta-lactamase originating from glaucous gull (Larus hyperboreus) in Svalbard

• Published in: Veterinary microbiology Vol. 171; no. 3-4; pp. 432 - 435
• Main Authors: Literak, Ivan, Manga, Ivan, Wojczulanis-Jakubas, Katarzyna, Chroma, Magdalena, Jamborova, Ivana, Dobiasova, Hana, Sedlakova, Miroslava Htoutou, Cizek, Alois
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.07.2014
• Subjects: Alle alle; Amino Acid Substitution - genetics; Animals; Antimicrobial resistance; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; beta-Lactamases - genetics; beta-Lactamases - metabolism; Birds; Charadriiformes - microbiology; Cloaca - microbiology; DNA Primers - genetics; Drug Resistance, Bacterial - genetics; Enterobacter cloacae; Enterobacter cloacae - enzymology; Enterobacter cloacae - genetics; Escherichia coli; Escherichia coli - genetics; Larus hyperboreus; Molecular Sequence Data; Norway; Polymerase Chain Reaction - veterinary; Polymorphism, Single Nucleotide - genetics; Salmonella; Sequence Analysis, DNA - veterinary; Svalbard; Wildlife; Norway; Svalbard; Birds; Bacteria; Antimicrobial resistance; Wildlife
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2014.02.015

We aimed at Escherichia coli and Enterobacter cloacae isolates resistant to cephalosporins and fluoroquinolones and Salmonella isolates in wild birds in Arctic Svalbard, Norway. Cloacal swabs of little auks (Alle alle, n=215) and samples of faeces of glaucous gulls (Larus hyperboreus, n=15) were examined. Inducible production of AmpC enzyme was detected in E. cloacae KW218 isolate. Sequence analysis of the 1146bp PCR product of the ampC gene from this isolate revealed 99% sequence homology with the blaACT-14 and blaACT-5 AmpC beta-lactamase genes. Four, respectively six of the identified single nucleotide polymorphisms generated amino acid substitutions in the amino acid chain. As the ampC sequence polymorphism in the investigated E. cloacae strain was identified as unique, we revealed a novel variant of the ampC beta-lactamase gene blaACT-23.