Immunoblot analysis of Schistosoma spindale excretory–secretory antigens with sera from naturally infected bovines

• Published in: Journal of Applied Animal Research Vol. 44; no. 1; pp. 210 - 214
• Main Authors: Lakshmanan, Bindu, Devada, K, Joseph, Siju, Radhika, R
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.01.2016; Taylor & Francis Ltd
• Subjects: Antigens; cattle; diagnostic techniques; excretory-secretory antigens; excretory-secretory products; immunoblot; Immunogenicity; Morbidity; mortality; Polypeptides; reproductive performance; Schistosoma; Schistosoma spindale; Schistosomosis; India
• ISSN: 0974-1844; 0971-2119; 0974-1844
• DOI: 10.1080/09712119.2015.1031770

Schistosoma spindale and Schistosoma indicum are the most prevalent causes of visceral schistosomosis among bovines of India. The infection causes morbidity, mortality, reduced productivity and poor subsequent reproductive performance and it is economically significant in producing animals. The high prevalence of S. spindale among slaughtered bovines of South India warrants the need to devise improved diagnostics for specific and timely detection of infection for appropriate treatment and control. The present study focused on analysing the excretory–secretory proteins of S. spindale and identification of the immunodominant polypeptide fractions of this antigen. The major polypeptide antigens corresponding to approximately 28 and 66 kDa size could be detected as highly immunogenic during natural infections in cattle. Consistent blotting pattern was observed with known schistosome positive bovine sera and specificity of the antigen was also ascertained with sera of amphistome and strongyle positive animals.