Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells

We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wa...

Full description

Saved in:
Bibliographic Details
Published inOptics express Vol. 20; no. 9; pp. 9673 - 9681
Main Authors Jang, Yunhun, Jang, Jaeduck, Park, YongKeun
Format Journal Article
LanguageEnglish
Published United States 23.04.2012
Subjects
Cells, Cultured
Colorimetry - instrumentation
Equipment Design
Equipment Failure Analysis
Erythrocyte Membrane - physiology
Erythrocyte Membrane - ultrastructure
Hemoglobins - analysis
Holography - instrumentation
Humans
Membrane Fluidity
Microscopy, Phase-Contrast - instrumentation
Molecular Imaging - instrumentation
Spectrum Analysis - instrumentation
Online AccessGet full text

Cover

More Information
Summary:We report a technique for simultaneous label-free quantification of cytoplasmic hemoglobin Hb concentration and dynamic membrane fluctuation in individual red blood cells (RBCs). Spectroscopic phase microscopy equipped with three different coherent laser sources and a color detector records three wavelength-dependent quantitative phase images in a single shot of a color-coded hologram. Using molecular specific dispersion, we demonstrate the extraction of Hb concentration and the dynamic membrane fluctuation from individual RBCs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.20.009673