Significance of Viable but Nonculturable Escherichia coli : Induction, Detection, and Control

• Published in: Journal of microbiology and biotechnology Vol. 27; no. 3; pp. 417 - 428
• Main Authors: Ding, Tian, Suo, Yuanjie, Xiang, Qisen, Zhao, Xihong, Chen, Shiguo, Ye, Xingqian, Liu, Donghong
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.03.2017
• Subjects: Animals; Bacterial Typing Techniques; Communicable Disease Control - methods; Environmental Microbiology; Escherichia coli - classification; Escherichia coli - physiology; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Infections - prevention & control; Escherichia coli Infections - transmission; Food Microbiology; Food Safety; Humans; Microbial Viability; Risk; Virulence - genetics; Virulence Factors - genetics; 생물학; resuscitation; detection methods; viable but nonculturable state; mechanism; Escherichia coli
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1609.09063

Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods, posing a significant and potential health risk. Therefore, controlling VBNC bacteria in food processing and the environment is of great importance. As the typical one of the gram-negatives, ( ) is a widespread foodborne and waterborne pathogenic bacterium and is able to enter into a VBNC state in extreme conditions (similar to the other gram-negative bacteria), including inducing factors and resuscitation stimulus. VBNC has the ability to recover both culturability and pathogenicity, which may bring potential health risk. This review describes the concrete factors (nonthermal treatment, chemical agents, and environmental factors) that induce into the VBNC state, the condition or stimulus required for resuscitation of VBNC , and the methods for detecting VBNC . Furthermore, the mechanism of genes and proteins involved in the VBNC is also discussed in this review.