STM and AFM images of nucleosome DNA under water

• Published in: Journal of biomolecular structure & dynamics Vol. 7; no. 2; p. 279
• Main Authors: Lindsay, S M, Nagahara, L A, Thundat, T, Knipping, U, Rill, R L, Drake, B, Prater, C B, Weisenhorn, A L, Gould, S A, Hansma, P K
• Format: Journal Article
• Language: English
• Published: England 01.10.1989
• Subjects: Animals; Cattle; DNA - ultrastructure; Microscopy, Electron, Scanning; Molecular Conformation; Nucleosomes - ultrastructure
• ISSN: 0739-1102
• DOI: 10.1080/07391102.1989.10507771

We have imaged DNA from the calf thymus nucleosome using a scanning tunneling microscope (STM) operated in water. The fragments are deposited onto the interface between a buffer solution and an epitaxially grown gold surface using an electrochemical tecnique. Most of the fragments are fairly straight, and when individual polymers can be identified, their length is consistent with the expected 146 basepairs (approximately 500 A). The resolution is often adequate to show signs of the 36 A helical pitch. Some images show a structure which appears to have abrupt kinks of the sort predicted by Crick and Klug (Nature 255, 530-533, 1975). In order to check that this shape is not a consequence of binding to underlying structure on the gold substrate, we have also made images of kinked structures using an atomic force microscope (AFM) with the DNA bound to glass.