Circulating Monocyte-Like Myeloid Derived Suppressor Cells and CD16 Positive Monocytes Correlate With Immunological Responsiveness of Tuberculosis Patients

• Published in: Frontiers in cellular and infection microbiology Vol. 12; p. 841741
• Main Authors: Amiano, Nicolás O, Pellegrini, Joaquín M, Morelli, María P, Martinena, Camila, Rolandelli, Agustín, Castello, Florencia A, Casco, Nicolás, Ciallella, Lorena M, de Casado, Graciela C, Armitano, Rita, Stupka, Juan, Gallego, Claudio, Palmero, Domingo J, García, Verónica E, Tateosian, Nancy L
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 14.03.2022
• Subjects: active infection; anti-TB treatment; Cellular and Infection Microbiology; Humans; intermediate and non-classical monocytes; Monocytes; Mycobacterium tuberculosis; Myeloid Cells; Myeloid-Derived Suppressor Cells; Tuberculosis; active infection; myeloid-derived suppressor cells; intermediate and non-classical monocytes; tuberculosis; anti-TB treatment
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2022.841741

Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). In this work, we studied the relationship between myeloid-derived suppressor cells (MDSC) and monocytes subsets with the immunological responsiveness of TB patients. Individuals with active TB were classified as low responders (LR-TB) or high responders (HR-TB) according to their T cell responses against a cell lysate of ( -Ag). Thus, LR-TB, individuals with severe disease, display a weaker immune response to compare to HR-TB, subjects with strong immunity against the bacteria. We observed that LR-TB presented higher percentages of CD16 positive monocytes as compared to HR-TB and healthy donors. Moreover, monocyte-like (M-MDSC) and polymorphonuclear-like (PMN-MDSC) MDSC were increased in patients and the proportion of M-MDSC inversely correlated with IFN-γ levels released after -Ag stimulation in HR-TB. We also found that LR-TB displayed the highest percentages of circulating M-MDSC. These results demonstrate that CD16 positive monocytes and M-MDSC frequencies could be used as another immunological classification parameter. Interestingly, in LR-TB, frequencies of CD16 positive monocytes and M-MDSC were restored after only three weeks of anti-TB treatment. Together, our findings show a link between the immunological status of TB patients and the levels of different circulating myeloid cell populations.