Germline‐specific expression of the Oct‐4/green fluorescent protein (GFP) transgene in mice

The Pic‐1, Oct‐1,2, Unc‐86 (POU) transcription factor Oct‐4 is specifically expressed in the germ cell line, and a previous study has indicated that the expression of the lacZ gene inserted into an 18 kb genomic fragment encompassing the Oct‐4 gene can come close to mimicking the endogenous embryoni...

Full description

Saved in:
Bibliographic Details
Published inDevelopment, growth & differentiation Vol. 41; no. 6; pp. 675 - 684
Main Authors Yoshimizu, Tomomi, Sugiyama, Noriyuki, De Felice, Mario, Yeom, Young Ii, Ohbo, Kazuyuki, Masuko, Kazue, Obinata, Masuo, Abe, Kuniya, Schöler, Hans R., Matsui, Yasuhisa
Format Journal Article
LanguageEnglish
Published Melbourne, Australia Blackwell Science Pty 01.12.1999
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:The Pic‐1, Oct‐1,2, Unc‐86 (POU) transcription factor Oct‐4 is specifically expressed in the germ cell line, and a previous study has indicated that the expression of the lacZ gene inserted into an 18 kb genomic fragment encompassing the Oct‐4 gene can come close to mimicking the endogenous embryonic expression pattern of Oct‐4 in transgenic mice. In the present study transgenic mice expressing green fluorescent protein (GFP) in the germ cell line were generated using the same Oct‐4 genomic fragments and the expression pattern was analyzed in detail through all stages of germ cell development. The GFP expressing primordial germ cells were first detected as early as 8.0 days post‐coitum (d.p.c.; early head fold stage) at the base of the allantois in living embryos. The GFP expression was thereafter found in both male and female germ cells at all developmental stages except in male germ cells after differentiating into type A spermatogonia in the postnatal testis. There was also a lower level of expression in female germ cells in the prophase of the first meiotic division. These transgenic mice therefore proved to be powerful tools for isolating living germ cells at various developmental stages to study their nature and to isolate new genes.
Bibliography:m60362@center.osaka‐u.ac.jp
Author to whom all correspondence should be addressed. E‐mail
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0012-1592
1440-169X
DOI:10.1046/j.1440-169x.1999.00474.x