Differential expression of complement proteins in cerebrospinal fluid from active multiple sclerosis patients

• Published in: Journal of cellular biochemistry Vol. 112; no. 7; pp. 1930 - 1937
• Main Authors: Li, Yun, Qin, Zhaoyu, Yang, Mingchong, Qin, Yanjiang, Lin, Chengzhao, Liu, Shilian
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.2011
• Subjects: 2-D DIGE; Adult; Biomarkers - cerebrospinal fluid; Case-Control Studies; Cerebrospinal fluid; Complement; Complement System Proteins - cerebrospinal fluid; Complement System Proteins - genetics; Electrophoresis, Gel, Two-Dimensional; Female; Gene Expression; Humans; Male; Middle Aged; Multiple sclerosis; Multiple Sclerosis - cerebrospinal fluid; Protein Interaction Mapping
• ISSN: 0730-2312; 1097-4644; 1097-4644
• DOI: 10.1002/jcb.23113

Multiple sclerosis (MS) is a demyelinating disease of the central nervous system with complex immunopathogenesis. Using the 2‐D DIGE technology, we separate CSF proteins from patients with active MS and control subjects. Three of the seven differential proteins identified were related with complement system, and the network analysis of the differential proteins revealed complement activation involvement in active MS. Complement C4b (gamma chain) was confirmed elevated by performing western blotting analysis (P < 0.01). The present results are an independent quantitative proteomic measure in CSF from active MS patients. The differential expression of the complement C4b and related proteins in CSF provides potential biomarkers as well as evidence for the involvement of complement activation in the pathogenesis of MS disease. J. Cell. Biochem. 112: 1930–1937, 2011. © 2011 Wiley‐Liss, Inc.