Establishment and characterization of a human primary prostate carcinoma cell line, HH870

• Published in: The Prostate Vol. 63; no. 1; pp. 91 - 103
• Main Authors: Selvan, Senthamil R., Cornforth, Andrew N., Rao, Nagesh P., Reid, Yvonne A., Schiltz, Patric M., Liao, Ray P., Price, David T., Heinemann, F. Scott, Dillman, Robert O.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2005; Wiley-Liss
• Subjects: androgens; Androgens - metabolism; Animals; Biological and medical sciences; Cell Culture Techniques - methods; Cell Division; Cell Line, Tumor - cytology; Cell Line, Tumor - transplantation; Chromosome Aberrations; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Male; Male genital diseases; Medical sciences; Mice; Mice, Nude; Neoplasm Transplantation; Nephrology. Urinary tract diseases; p53; primary tumor cell line; prostate cancer; Prostatic Neoplasms - pathology; PTEN; tumorigenic cell line; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Andrology; Prostate carcinoma; Nephrology; Prostate disease; androgens; PTEN; p53; Characterization; Primitive; TP53 Gene; Established cell line; Primary; PTEN Gene; primary tumor cell line; Male genital diseases; Tumor cell; Tumor suppressor gene; Human; Urinary system disease; Androgen; Gynecology; Tumorigenicity; Malignant tumor; tumorigenic cell line; Cell line; Sex steroid hormone; Prostate cancer
• ISSN: 0270-4137; 1097-0045
• DOI: 10.1002/pros.20162

BACKGROUND Development of new therapeutic modalities for human prostate carcinoma has been impeded by a lack of adequate in vitro and in vivo models. Most in vitro studies have been carried out using a limited number of human prostate cancer cell lines that are mostly derived from metastatic tumors sites or are immortalized. METHODS Characterization of the prostate cancer cell line, HH870, included description of morphology, determination of doubling time, response to androgens, immunocytochemistry, and immunoblotting of proteins known to be associated with prostate carcinoma, karyotyping, fluorescence in situ hybridization (FISH), DNA profiling, and growth as xenograft in athymic rodents. RESULTS HH870 expresses various epithelial marker antigens that correlate with known basic immunostaining profiles of prostate adenocarcinoma, although the cell line does not express PSA, PSMA, or PAP. HH870 exhibits complex chromosomal abnormalities and harbors no immortalizing HPV, BKV, JCV, and SV40 DNA. CONCLUSIONS We report the successful establishment and characterization of a new long‐term primary human prostate tumor cell line HH870. © 2004 Wiley‐Liss, Inc.