Emodin Improves Lipopolysaccharide-Induced Microcirculatory Disturbance in Rat Mesentery

• Published in: Microcirculation (New York, N.Y. 1994) Vol. 20; no. 7; pp. 617 - 628
• Main Authors: Li, Ang, Dong, Lei, Duan, Mei-Li, Sun, Kai, Liu, Yu-Ying, Wang, Ming-Xia, Deng, Jing-Na, Fan, Jing-Yu, Wang, Bao-En, Han, Jing-Yan
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.10.2013; Wiley Subscription Services, Inc
• Subjects: activator protein-1; adhesion molecules; Animals; Emodin - pharmacology; Endotoxemia - chemically induced; Endotoxemia - drug therapy; Endotoxemia - metabolism; Endotoxemia - physiopathology; Humans; Intercellular Adhesion Molecule-1 - biosynthesis; Leukocytes; Lipopolysaccharides - toxicity; Male; Mesentery - blood supply; Mesentery - metabolism; Mesentery - pathology; Mesentery - physiopathology; Microcirculation - drug effects; nuclear factor kappa B p65; Peroxidase - biosynthesis; Protein Kinase Inhibitors - pharmacology; Rats; Rats, Wistar; Regional Blood Flow - drug effects; Rheum palmatum; Rodents; toll-like receptor 4; Toll-Like Receptor 4 - biosynthesis; Transcription Factor AP-1 - biosynthesis; Transcription Factor RelA - biosynthesis; Rheum palmatum; adhesion molecules; toll-like receptor 4; activator protein-1; nuclear factor kappa B p65
• ISSN: 1073-9688; 1549-8719
• DOI: 10.1111/micc.12061

Objective Sepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum, a Chinese herb that is widely used in China for treatment of endotoxemia‐related diseases. This study intended to examine the effect of Emodin on LPS‐induced rat mesenteric microcirculatory disturbance and the underlying mechanisms. Methods The male Wistar rats received LPS (5 mg/kg/hr) for 90 min, with or without administration of Emodin (10 mg/kg/hr) by enema 30 min before (pre‐treatment) or after (post‐treatment) LPS infusion, and the dynamics of mesenteric microcirculation were determined by inverted intravital microscopy. Expression of adhesion molecules and TLR4, NF‐κB p65, ICAM‐1, MPO, and AP‐1 in mesentery tissue was evaluated by flow cytometry and Western‐blot, respectively. Results Pre or post‐treatment with Emodin significantly ameliorated LPS‐induced leukocyte emigration, reactive oxygen species production and albumin leakage, and the expression of TLR4, NF‐κB p65, ICAM‐1, MPO and AP‐1 in mesentery. Conclusions These results demonstrate the beneficial role of Emodin in attenuating the LPS‐induced microcirculatory disturbance, and support the use of Emodin for patients with endotoxemia.