Localization of the Gene Encoding the Human Heat Shock Cognate Protein, HSP73, to Chromosome 11

• Published in: Genomics (San Diego, Calif.) Vol. 29; no. 1; pp. 266 - 268
• Main Authors: Tavaria, Michael, Gabriele, Tim, Anderson, Robin L., Mirault, Marc-Edouard, Baker, Elizabeth, Sutherland, Grant, Kola, Ismail
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1995; Elsevier
• Subjects: Animals; Biological and medical sciences; Blotting, Southern; Blotting, Western; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Cell Line; CHO Cells; Chromosome Mapping; Chromosomes, Human, Pair 11; Chromosomes, Human, Pair 5; Chromosomes, Human, Pair 6; Chromosomes, Human, Pair 9; Classical genetics, quantitative genetics, hybrids; Cricetinae; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Hominidae - genetics; Hot Temperature; HSC70 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - genetics; Human; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Male; Molecular Weight; Multigene Family; Genetic mapping; Human; Multigene family; Gene; C11-Chromosome; Heat shock protein
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.1242

The heat shock cognate protein HSP73 (or HSC70) is a member of the HSP70 multigene family. This protein has several functions, including binding to nascent polypeptides to facilitate correct folding and the uncoating of clathrin-coated vesicles. Analysis of somatic cell hybrids by two-dimensional protein gel electrophoresis revealed the presence of a 73-kDa protein in two hybrids containing human chromosomes 5, 6, 9, and 11 in common. Using Western blot analysis, we demonstrate that this protein is a member of the HSP70 family and, by Southern blot analysis, that the HSP73 gene is located on human chromosome 11. Fluorescence in situ hybridization further localized HSP73 to the region 11q23.3-q25. This region is involved in a number of genetic rearrangements and is associated with several well-characterized tumors.