Potential antithrombotic effect of two new phenylpropanoid sucrose esters and other secondary metabolites of Canna indica L. rhizome

• Published in: Natural product research Vol. 38; no. 6; pp. 897 - 905
• Main Authors: Nguyen, Thi Van Anh, Nguyen, Thi Minh Hang, Le, Hong Luyen, Bui, Duc Huy
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 18.03.2024; Taylor & Francis Ltd
• Subjects: Acetic acid; Acids; Adenosine diphosphate; anticoagulant; Anticoagulants; antiplatelet aggregation; Aquatic plants; Caffeic acid; Canna indica; Coagulation; Esters; Ethyl acetate; Ferulic acid; heart diseases; Metabolites; NMR; Nuclear magnetic resonance; phytochemicals; Platelet aggregation; Prothrombin; Rhizomes; Rosmarinic acid; Secondary metabolites; Sucrose; Sucrose esters; Thromboembolism; Thrombosis; Canna indica; phytochemicals; anticoagulant; antiplatelet aggregation; heart diseases
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2023.2262712

Canna indica L. has been traditionally used to treat various diseases. Based on previously reported antithrombotic effect for this plant, two new phenylpropanoid sucrose esters (canindicoside A (1) and canindicoside B (2)) and seven known compounds: nepetoidin B (3), caffeic acid (4), ferulic acid (5), (R)-(+)-rosmarinic acid (6), isorinic acid (7), (S)-(-)-rosmarinic acid (8) and (S)-(-)-rosmarinic acid methyl ester (9) were isolated from the ethyl acetate extract. Compounds were elucidated by NMR and MS spectroscopic methods. The antiplatelet effect was evaluated using turbidimetric method. Anticoagulant activity was examined by measuring activated partial thromboplastine time (APTT), prothrombin time, and thrombine time (TT). It was shown for the first time that both new phenylpropanoid sucrose esters 1 and 2, 7 and 9 displayed dose-dependent antiplatelet effects. 2 and 9 had the highest inhibitory activity on both adenosine diphosphate (ADP)- and collagen-induced platelet aggregation. Moreover, 1, 7 and 9 also exhibited anticoagulant activity. At 0.4 mg/mL, both 1 and 7 prolonged APTT compared to the negative control (p < 0.05), suggesting the possible inhibitory impact on the intrinsic coagulation pathway. Moreover, 9 at 0.4 mg/mL exerted higher TT values than the negative control (p < 0.05). C. indica and its bioactive phytochemicals are potential candidates for development of anti-thrombosis therapy.