Limonoids with neuroprotective activity from the stems of Clausena emarginata

• Published in: Journal of asian natural products research Vol. 18; no. 10; pp. 928 - 937
• Main Authors: Ouyang, Guo-Qing, Li, Chuang-Jun, Yang, Jing-Zhi, Ma, Jie, Li, Li, Peng, Ying, Wang, Xiao-Liang, Zhang, Dong-Ming
• Format: Journal Article
• Language: English
• Published: ABINGDON Taylor & Francis 01.10.2016; Taylor & Francis Ltd
• Subjects: Chemistry; Chemistry, Applied; Chemistry, Medicinal; Clausena - chemistry; Clausena emarginata; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Glutamic Acid - pharmacology; Humans; Life Sciences & Biomedicine; Limonins - chemistry; Limonins - isolation & purification; Limonins - pharmacology; limonoids; Molecular Structure; Neuroblastoma - chemically induced; neuroprotective; Neuroprotective Agents - pharmacology; Pharmacology & Pharmacy; Physical Sciences; Plant Sciences; Plant Stems - chemistry; Rutaceae; Science & Technology; SEEDS; Clausena emarginata; limonoids; neuroprotective; Rutaceae; ALKALOIDS
• ISSN: 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2016.1193491

Two new limonoids, clauemargines M-N (1-2), together with five known compounds (3-7), were isolated from the stems of Clausena emarginata, and compounds 6 and 7 were gained from this plant for the first time. Their structures were established and elucidated on the basis of comprehensive spectroscopic analysis. The absolute configurations of 1-2 were further determined by the octant rule of saturated cyclic ketone. Compounds 1, 2, 4, and 5 showed moderate neuroprotective effects against L-glutamic acid-induced cellular damage in human neuroblastoma SK-N-SH cells at 10 μM.