Progesterone receptor immunoreactivity at the maternofetal interface of first trimester pregnancy: a study of the trophoblast population

• Published in: Human reproduction (Oxford) Vol. 11; no. 2; pp. 413 - 419
• Main Authors: Wang, Jie-dong, Jian-bao, Zhu, Fu, Yao, Shi, Wen-liang, Qiao, Gen-mei, Wang, You-qu, Chen, Jie, Zhu, Peng-di
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.02.1996
• Subjects: Adult; Animals; Antibodies, Monoclonal; Biological and medical sciences; Chorionic Villi - metabolism; Decidua - blood supply; Decidua - metabolism; Embryology: invertebrates and vertebrates. Teratology; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Female; Fetal membranes; first trimester pregnancy; Fundamental and applied biological sciences. Psychology; General aspects. Development. Fetal membranes; Humans; iirimunohistochemistry; Immunohistochemistry - methods; Pregnancy; Pregnancy Trimester, First; progesterone receptor; Rats; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; Staining and Labeling; trophoblast; Trophoblasts - metabolism; iirimunohistochemistry; trophoblast; progesterone receptor; first trimester pregnancy; Human; Trophoblaste; Fetal membrane; Distribution; Female; Early pregnancy; Progesterone; Sex steroid hormone; Ovarian hormone; Hormonal receptor
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/HUMREP/11.2.413

The aim of oar study was to localize oestrogen receptor (ER) and progesterone receptor (PR) in the trophoblast population at the maternofetal interface in early pregnancy. Rat monoclonal antibodies to human ER and PR were used to study 44 cases of chorionic villi and 82 cases of decidua using an immunocytochemical method. The PR-immunoreactive products were localized in the nuclei of syncytiotrophoblast and cytotrophoblast cells of the villi. Specific staining was also present in the cytoplasm. Villous stroma and vessels were stained occasionally. Using cytokeratin staining in an adjacent section or double staining with PR and cytokeratin, the distribution of invading trophoblast cells and their PR expression were examined. In decidual stroma, a type of interstitial cell was identified which simultaneously expressed cytokeratin and PR in the cytoplasm, indicating that the invading trophoblast cells may express PR. All extravillous populations at the interface were positive for PR, including the syncytial lining of the decidual surface, the cytotrophoblast column, the cytotrophoblast shell and the interstitial trophoblast. The immunoreactivity of PR was also localized in the nuclei of vascular endothelial cells, whereas Factor VIII was localized in the cytoplasm of the same cells, thus confirming their endothelial nature. In contrast to PR, little ER could be detected in the trophoblast cell population using anti-ER antibody D75 in our study.