Acquisition of cross-polarized diffraction images and study of blurring effect by one time-delay-integration camera

• Published in: Applied optics. Optical technology and biomedical optics Vol. 54; no. 16; p. 5223
• Main Authors: Wang, He, Feng, Yuanming, Sa, Yu, Ma, Yuxiang, Lu, Jun Q, Hu, Xin-Hua
• Format: Journal Article
• Language: English
• Published: United States 01.06.2015
• Subjects: Cell Tracking - instrumentation; Cell Tracking - methods; Equipment Design; Equipment Failure Analysis; Flow Cytometry - instrumentation; Flow Cytometry - methods; Humans; Image Enhancement - instrumentation; Image Enhancement - methods; Image Interpretation, Computer-Assisted - instrumentation; Image Interpretation, Computer-Assisted - methods; K562 Cells; MCF-7 Cells; Microscopy, Fluorescence - instrumentation; Microscopy, Fluorescence - methods; Microscopy, Phase-Contrast - instrumentation; Microscopy, Phase-Contrast - methods; Refractometry - instrumentation; Refractometry - methods; Reproducibility of Results; Sensitivity and Specificity; Subcellular Fractions - ultrastructure; Systems Integration
• ISSN: 2155-3165
• DOI: 10.1364/AO.54.005223

Blurred diffraction images acquired from flowing particles affect the measurement of fringe patterns and subsequent analysis. An imaging unit with one time-delay-integration (TDI) camera has been developed to acquire two cross-polarized diffraction images. It was shown that selected elements of Mueller matrix of single scatters can be imaged with pixel matching precision in this configuration. With the TDI camera, the effect of blurring on imaging of scattered light propagating along the side directions was found to be much more significant for biological cells than microspheres. Despite blurring, classification of MCF-7 and K562 cells is feasible since the effect has similar influence on extracted image parameters. Furthermore, image blurring can be useful for analysis of the correlations among texture parameters for characterization of diffraction images from single cells. The results demonstrate that with one TDI camera the polarization diffraction imaging flow cytometry can be significantly improved and angular distribution of selected Mueller matrix elements can be accurately measured for rapid and morphology-based assay of particles and cells without fluorescent labeling.