Anthocyanins of Blue Corn and Tortilla Arrest Cell Cycle and Induce Apoptosis on Breast and Prostate Cancer Cells

• Published in: Nutrition and cancer Vol. 72; no. 5; pp. 768 - 777
• Main Authors: Herrera-Sotero, Mónica Y., Cruz-Hernández, Carlos D., Oliart-Ros, Rosa M., Chávez-Servia, José L., Guzmán-Gerónimo, Rosa I., González-Covarrubias, Vanessa, Cruz-Burgos, Marian, Rodríguez-Dorantes, Mauricio
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03.07.2020; Taylor & Francis Ltd
• Subjects: Adjuvants; Androgen receptors; Androgens; Anthocyanins; Anthocyanins - pharmacology; Apoptosis; Apoptosis - drug effects; Biotechnology; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cell cycle; Cell Cycle Checkpoints - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Cell viability; Column chromatography; Corn; Estrogens; Female; Flavonoids - pharmacology; Flow cytometry; G1 phase; Humans; Male; Nocodazole; Progesterone; Prostate cancer; Prostatic Neoplasms - drug therapy; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Receptors; Receptors, Androgen - metabolism; Regional development; Side effects; Tumor cell lines; Tumors; Zea mays - chemistry
• ISSN: 0163-5581; 1532-7914
• DOI: 10.1080/01635581.2019.1654529

Background: Breast and prostate cancer are frequently diagnosed neoplasias in women and men around the world. The signaling of the androgen receptor (AR) influences the development of both tumors. Since therapies focused to block the receptor's activity have not been fully effective, and have shown side effects, therapies based on natural compounds are promissory complementary alternatives in its treatment. Objective: The aim of this study was to determine the effect of anthocyanins from blue corn in cancer cell lines. Methods: We analyzed the antiproliferative effect of anthocyanins from raw and alkali-processed (tortillas) Mixteco blue corn in breast and prostate cancer cell lines MDA-MB-453 (subtype: triple negative) and LNCaP using methyltiazlyl-tetrazolium (MTT) and flow cytometry (FCM). The combination of anthocyanins and 2-amino-N-quinolin-8-yl-benzenesulfonamide (QBS) or nocodazole also were evaluated. The anthocyanins were isolated trough column chromatography (XAD-7). Results: Our results demonstrated that anthocyanin specially the ones obtained from tortillas, decreased cell viability and arrested cell cycle in G1 phase inducing apoptosis. Cytometry analysis shows an increased effect on apoptosis of MDA-MB-453 and LNCaP cells when tortilla anthocyanins and QBS were combined. Conclusions: This is the first report that suggest that anthocyanins from blue corn have an effect in cell cycle and viability so they could serve as adjuvants for breast and prostate cancer therapies and may prompt to deepen investigations to decipher its molecular properties. Abbreviations AR Androgen Receptor CIDIIR Interdisciplinary Center for Research on Integral Regional Development DHT 5α-Dihydrotestosterone ER Estrogen Receptor PR Progesterone Receptor QBS Amino-N-quinolin-8-yl-benzenesulfonamide