Effects of metal ions on thermal stabilities of DNA duplexes containing homo- and heterochiral mismatched base pairs: comparison of internal and terminal substitutions

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 39; no. 1-3; pp. 310 - 321
• Main Authors: Funai, Tatsuya, Adachi, Nahomi, Aotani, Megumi, Wada, Shun-ichi, Urata, Hidehito
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 20.02.2020; Taylor & Francis Ltd
• Subjects: Base pair mismatch; Base pairs; Deoxyribonucleic acid; DNA; fraying; heterochiral DNA; Metal ions; metal-mediated base pair; Base pair mismatch; metal-mediated base pair; fraying; heterochiral DNA
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1080/15257770.2019.1658116

The effects of metal ions on the stabilities of duplexes containing a D -homochiral and heterochiral mismatched base pairs were studied. In some duplexes containing an internal mismatched base pair, significant stabilization by Hg II and Ag I ions was observed. While, in duplexes containing a terminal mismatched base pair, only the duplexes containing T-T and L T-T mispairs were significantly stabilized by Hg II ions, and the stabilities of the duplexes containing T-T and L T-T mispairs exceeded those of the corresponding homochiral matched duplex. The results suggest that the formation of homo- and heterochiral T-Hg II -T base pairs at duplex termini would be useful for the thermal and enzymatic stabilization of DNA-based nanodevice.